适体生物识别触发的 DNA zyme 释放和 Exo III 辅助的靶标循环用于庆大霉素抗生素的超灵敏均相比色生物分析。
Aptamer biorecognition-triggered DNAzyme liberation and Exo III-assisted target recycling for ultrasensitive homogeneous colorimetric bioassay of kanamycin antibiotic.
机构信息
Hubei Key Laboratory of Pollutant Analysis & Reuse Technology, Department of Chemistry, Hubei Normal University, Huangshi 435002, P. R. China.
出版信息
Chem Commun (Camb). 2019 Apr 7;55(27):3959-3962. doi: 10.1039/c8cc10107h. Epub 2019 Mar 15.
PMID:30874255
Abstract
A novel peroxidase-mimicking DNAzyme-based colorimetric homogenous bioassay is developed for kanamycin detection. Aptamer biorecognition triggers DNAzyme liberation from DNA duplexes for "off-on" signal transduction. The special hairpin structure of the aptamer-kanamycin biocomposite enables Exo III-assisted target recycling for signal amplification.
摘要
一种新型基于过氧化物酶模拟的 DNA 酶比色均相生物测定法被开发用于庆大霉素检测。适体生物识别触发 DNA 酶从 DNA 双链体中释放，用于“开-关”信号转导。适体-庆大霉素复合物的特殊发夹结构使 Exo III 能够辅助靶标循环用于信号放大。
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