基于 DNA zyme 的 DNA 行走机器用于超灵敏荧光适体检测卡那霉素。

DNAzyme-powered DNA walking machine for ultrasensitive fluorescence aptasensing of kanamycin.

机构信息

Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province and Engineering Research Center of High Value Utilization of Western China Fruit Resources, College of Food Engineering and Nutritional Science, Shaanxi Normal University, Xi'an, 710119, China.

Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry & Chemical Engineering, Shaanxi Normal University, Xi'an, 710119, China.

出版信息

Mikrochim Acta. 2020 Nov 28;187(12):678. doi: 10.1007/s00604-020-04638-4.

DOI:10.1007/s00604-020-04638-4

PMID:33247409

Abstract

A DNAzyme-powered DNA walking machine was constructed to develop the fluorescence aptasensing for sensitive detection of kanamycin. The aptamer for kanamycin is partially hybridized with complementary DNA (cDNA) modified on magnetic beads (MBs). The specific interaction of target and aptamer triggered the cDNA to be free tentatively, which captured walker DNA. Then the autonomous motion of DNA walker on MBs surface was propelled via DNAzyme digestion of recognition sites. The signal probe was separated, and the amplified fluorescence signal was achieved by the accumulation of the signal probe. Kanamycin was used as a model analyte, and the developed assay achieves a detection limit of 0.00039 ng·mL (S/N = 3) within a linear detection range from 0.001 to 2000 ng·mL. This aptasensing strategy can be extended for detection of other antibiotics by adapting corresponding target recognition aptamer sequence. Graphical abstract The fluorescence aptasensing for sensitive detection of kanamycin based on DNAzyme-powered DNA walking machine was constructed.

摘要

基于 DNA zyme 驱动的 DNA 行走机器的荧光适体传感用于灵敏检测卡那霉素。卡那霉素的适体与修饰在磁性珠 (MBs) 上的互补 DNA (cDNA) 部分杂交。目标与适体的特异性相互作用触发 cDNA 暂时游离，从而捕获行走 DNA。然后，通过识别位点的 DNAzyme 消化来推动 DNA 行走器在 MBs 表面上的自主运动。信号探针被分离，并通过信号探针的积累实现放大的荧光信号。以卡那霉素为模型分析物，所开发的测定法在 0.001 至 2000 ng·mL 的线性检测范围内实现了 0.00039 ng·mL（S/N = 3）的检测限。通过适应相应的靶标识别适体序列，该适体传感策略可扩展用于检测其他抗生素。