Duval D, Lynde P, Hatzfeld A, Hatzfeld J
Biochim Biophys Acta. 1986 Jul 11;887(2):204-13. doi: 10.1016/0167-4889(86)90056-x.
Since the presence of serum in culture media has been shown to alter prostaglandin production, as well as to interfere with the action of anti-inflammatory drugs, we have studied the effect of dexamethasone, a potent steroidal anti-inflammatory drug, on the metabolism of arachidonic acid by human monocyte-like cells (U937) grown in a fully defined medium. Under these culture conditions, dexamethasone (10(-6) M, 24 h) induced a marked stimulation of the release of unmetabolized arachidonic acid into the culture medium. The steroid also induced an inhibition of cell proliferation which became significant only after 48 h of treatment. The accumulation of arachidonic acid in the medium after steroid treatment was associated with a significant inhibition of cell acyltransferase activity, suggesting that steroids may also act upon arachidonic acid metabolism at sites other than those of phospholipase activity.
由于已证明培养基中血清的存在会改变前列腺素的产生，以及干扰抗炎药物的作用，我们研究了强效甾体抗炎药地塞米松对在完全限定培养基中生长的人单核细胞样细胞（U937）花生四烯酸代谢的影响。在这些培养条件下，地塞米松（10⁻⁶ M，24小时）显著刺激未代谢花生四烯酸释放到培养基中。该甾体还诱导细胞增殖受到抑制，仅在处理48小时后才变得显著。甾体处理后培养基中花生四烯酸的积累与细胞酰基转移酶活性的显著抑制相关，这表明甾体可能也在磷脂酶活性位点以外的其他位点作用于花生四烯酸代谢。
