Errasfa M, Rothhut B, Fradin A, Billardon C, Junien J L, Bure J, Russo-Marie F
Biochim Biophys Acta. 1985 Nov 20;847(2):247-54. doi: 10.1016/0167-4889(85)90027-8.
Human embryonic skin fibroblasts in culture produce pro-inflammatory lipid mediators and all types of prostanoids. When these cells were treated with the anti-inflammatory steroid, dexamethasone, prostaglandin production was inhibited. This phenomenon required glucocorticoid receptor occupancy and mRNA and protein synthesis. The inhibitory effect was prevented by treating the cells with a monoclonal antibody, BF 26, raised against renocortin, a lipocortin-like protein formed in rat kidney medulla interstitial cells in culture. When the proteins present in the supernatants and the cell pellets derived from control and dexamethasone-treated cells were analyzed for their ability to inhibit phospholipase A2, four inhibitory peaks, at 45, 30, 15 kDa and one peak under 12 kDa, were found in the supernatants of control and dexamethasone-treated cells, whereas one single inhibitory peak at 15 kDa was found in the cell pellets. The antiphospholipase activity was much greater in dexamethasone-treated cells than in control cells. These results suggest that preformed lipocortin exists in human cells and that lipocortin is synthesized and released under glucocorticoid treatment.
培养的人胚胎皮肤成纤维细胞会产生促炎性脂质介质和各类前列腺素。当用抗炎类固醇地塞米松处理这些细胞时，前列腺素的产生受到抑制。这种现象需要糖皮质激素受体被占据以及mRNA和蛋白质合成。用针对肾皮质素（一种在培养的大鼠肾髓质间质细胞中形成的脂皮质素样蛋白）产生的单克隆抗体BF 26处理细胞，可阻止这种抑制作用。当分析对照细胞和地塞米松处理细胞的上清液及细胞沉淀中存在的蛋白质抑制磷脂酶A2的能力时，在对照细胞和地塞米松处理细胞的上清液中发现了4个抑制峰，分子量分别为45 kDa、30 kDa、15 kDa以及一个低于12 kDa的峰，而在细胞沉淀中仅发现一个15 kDa的单一抑制峰。地塞米松处理细胞的抗磷脂酶活性比对照细胞高得多。这些结果表明人细胞中存在预先形成的脂皮质素，并且脂皮质素在糖皮质激素处理下会合成并释放。
