Functional Role of Basolateral ClC-2 Channels in the Regulation of Duodenal Anion Secretion in Mice.

BACKGROUND

Although ClC-2 channels are important in colonic Cl secretion, it is unclear about their roles in small intestinal anion secretion. Therefore, we sought to examine whether ClC-2 channels play important roles in anion secretion, particularly duodenal bicarbonate secretion (DBS).

METHODS

Duodenal mucosae from mice were stripped of seromuscular layers and mounted in Ussing chambers. Both duodenal short-circuit current (I) and HCO secretion in vitro were simultaneously recorded. DBS in vivo was measured by a CO-sensitive electrode.

RESULTS

Lubiprostone, a selective ClC-2 activator, concentration-dependently increased both duodenal I and DBS only when applied basolaterally, but not when applied apically. Removal of extracellular Cl abolished lubiprostone-induced duodenal I, but did not alter HCO secretion even in the presence of DIDS, a Cl/HCO exchanger inhibitor. However, further addition of glibenclamide, a CFTR channel blocker, abolished lubiprostone-evoked HCO secretion. Moreover, lubiprostone-induced HCO secretion was impaired in CFTR mice compared to wild-type littermates. Luminal perfusion of duodenal lumen with lubiprostone did not alter basal DBS in vivo, but lubiprostone (i.p.) was able to induce DBS, which was also significantly inhibited by Cd, a ClC-2 channel blocker. [Ca] level, Ca-activated K channel- and cAMP-mediated duodenal I, and HCO secretion were unchanged by lubiprostone.

CONCLUSIONS

We have provided the first evidence for the novel functional role of basolateral ClC-2 channels in the regulation of duodenal anion secretion.