Epp Laura S, Zimmermann Heike H, Stoof-Leichsenring Kathleen R
Alfred Wegener Institute Helmholtz Centre for Polar and Marine Research, Polar Terrestrial Environmental Systems, Potsdam, Germany.
Department of Biology, University of Konstanz, Konstanz, Germany.
Methods Mol Biol. 2019;1963:31-44. doi: 10.1007/978-1-4939-9176-1_5.
Environmental DNA preserved in sediments is rapidly gaining importance as a tool in paleoecology. Sampling procedures for sedimentary ancient DNA (sedaDNA) have to be well planned to ensure clean subsampling of the inside of sediment cores and avoid introducing contamination. Additionally, ancient DNA extraction protocols may need to be optimized for the recovery of DNA from sediments, which may contain inhibitors. Here we describe procedures for subsampling both nonfrozen and frozen sediment cores, and we describe an efficient method for ancient DNA extraction from such samples.
保存在沉积物中的环境DNA作为古生态学中的一种工具正迅速变得重要起来。沉积古DNA（sedaDNA）的采样程序必须精心规划，以确保对沉积物岩芯内部进行干净的子采样，并避免引入污染。此外，可能需要优化古代DNA提取方案，以从可能含有抑制剂的沉积物中回收DNA。在这里，我们描述了对未冷冻和冷冻沉积物岩芯进行子采样的程序，并描述了从这些样本中提取古代DNA的有效方法。
