Cawthron Institute, Private Bag 2, Nelson 7042, New Zealand; School of Biological Sciences, University of Auckland, Auckland 1142, New Zealand.
Northcott Research Consultants, 20 River Oaks Place, Hamilton 3200, New Zealand.
Ecotoxicol Environ Saf. 2019 Jun 15;174:611-617. doi: 10.1016/j.ecoenv.2019.03.022. Epub 2019 Mar 12.
Well validated and reliable biological assays using local and native species are required to characterise the impacts of pollution on ecosystem health. We identified a native estuarine pelagic copepod species suitable for assessing the ecotoxicological impact of anthropogenic contaminants. Gladioferens pectinatus fulfilled the necessary-selection criteria of: wide distribution and abundance across New Zealand estuaries, ease of maintenance in the laboratory, short life cycle, sensitivity to toxicants with different modes of action, and providing reproducibility of biological response to toxicants. Measured endpoints were survival and larval development rate for the nauplii, and survival, realized offspring and total potential offspring for adults. LC values for the survival of G. pectinatus exposed to copper, phenanthrene and chlorpyrifos were 170 (143-193), 181.3 (131.3-231.3) and 4.3 (3.8-4.9) µg/L, respectively. The most sensitive chronic endpoint identified for G. pectinatus was the larval development rate, with EC values of 49.8 (45-55.3), 31.3 (24.8-44.7) and 1.97 (1.6-2.31) µg/L for copper, phenanthrene and chlorpyrifos, respectively. The acute and chronic responses obtained for G. pectinatus against the three reference toxicants are comparable with those reported for other copepod species and confirm its sensitivity and suitability to assess the toxicity of New Zealand estuarine samples.
需要使用当地和本地物种进行经过充分验证和可靠的生物测定，以描述污染对生态系统健康的影响。我们确定了一种适合评估人为污染物对生态毒理学影响的本地沿海浮游桡足类物种。Gladiopferens pectinatus 符合必要的选择标准：在新西兰的各个河口广泛分布且丰富，易于在实验室中维持，生命周期短，对具有不同作用方式的毒物敏感，并且可以对毒物的生物反应提供重现性。测量的终点是无节幼体的存活率和幼虫发育率，以及成体的存活率、实际后代和总潜在后代。暴露于铜、菲和毒死蜱的 G.pectinatus 的 LC 值分别为 170（143-193）、181.3（131.3-231.3）和 4.3（3.8-4.9）µg/L。确定的 G.pectinatus 最敏感的慢性终点是幼虫发育率，铜、菲和毒死蜱的 EC 值分别为 49.8（45-55.3）、31.3（24.8-44.7）和 1.97（1.6-2.31）µg/L。G.pectinatus 对三种参考毒物的急性和慢性反应与其他桡足类物种的报告相当，证实了其对评估新西兰河口样本毒性的敏感性和适用性。
