Faculty of Agronomy, Jilin Agricultural University, Changchun 130118, China.
Institute of Agricultural Biotechnology, Jilin Academy of Agricultural Sciences, Changchun 130033, China.
Int J Mol Sci. 2019 Mar 14;20(6):1294. doi: 10.3390/ijms20061294.
A novel rice lesion mimic mutant (LMM) was isolated from the mutant population of Japonica rice cultivar Hitomebore generated by ethyl methane sulfonate (EMS) treatment. Compared with the wild-type (WT), the mutant, tentatively designated E40, developed necrotic lesions over the whole growth period along with detectable changes in several important agronomic traits including lower height, fewer tillers, lower yield, and premature death. To understand the molecular mechanism of mutation-induced phenotypic differences in E40, a proteomics-based approach was used to identify differentially accumulated proteins between E40 and WT. Proteomic data from isobaric tags for relative and absolute quantitation (iTRAQ) showed that 233 proteins were significantly up- or down-regulated in E40 compared with WT. These proteins are involved in diverse biological processes, but phenylpropanoid biosynthesis was the only up-regulated pathway. Differential expression of the genes encoding some candidate proteins with significant up- or down-regulation in E40 were further verified by qPCR. Consistent with the proteomic results, substance and energy flow in E40 shifted from basic metabolism to secondary metabolism, mainly phenylpropanoid biosynthesis, which is likely involved in the formation of leaf spots.
一个新的水稻病斑模拟突变体（LMM）是从 EMS 处理的粳稻品种“Hitomebore”的突变体群体中分离出来的。与野生型（WT）相比，暂定名为 E40 的突变体在整个生长过程中会出现坏死斑，同时几个重要的农艺性状也发生了可检测的变化，包括株高降低、分蘖减少、产量降低和过早死亡。为了了解突变引起的 E40 表型差异的分子机制，我们使用基于蛋白质组学的方法来鉴定 E40 和 WT 之间差异积累的蛋白质。iTRAQ 的蛋白质组学数据显示，与 WT 相比，E40 中有 233 种蛋白质显著上调或下调。这些蛋白质参与了多种生物学过程，但苯丙烷生物合成是唯一上调的途径。通过 qPCR 进一步验证了 E40 中一些候选蛋白基因表达的差异，这些蛋白基因的表达显著上调或下调。与蛋白质组学结果一致的是，E40 中的物质和能量流从基础代谢转向次生代谢，主要是苯丙烷生物合成，这可能与叶片斑点的形成有关。
