Institute of Crop Science and Zhejiang Key Laboratory of Crop Germplasm, Zhejiang University, Hangzhou 310058, China.
Institute of Crop Science and Resource Conservation (INRES), Abiotic Stress Tolerance in Crops, University of Bonn, 53115 Bonn, Germany.
Int J Mol Sci. 2017 Sep 14;18(9):1975. doi: 10.3390/ijms18091975.
To uncover the alleviation mechanism of quinclorac stress by salicylic acid (SA), leaf samples of ssp. under quinclorac stress with and without SA pre-treatment were analyzed for transcriptional and proteomic profiling to determine the differentially expressed genes (DEGs) and proteins (DEPs), respectively. Results showed that quinclorac stress altered the expression of 2207 DEGs (1427 up-regulated, 780 down-regulated) and 147 DEPs (98 down-regulated, 49 up-regulated). These genes and proteins were enriched in glutathione (GSH) metabolism, porphyrin and chlorophyll metabolism, the biosynthesis of secondary metabolites, glyoxylate and dicarboxylate metabolism, and so on. It also influenced apetala2- ethylene-responsive element binding protein (AP2-EREBP) family, myeloblastosis (MYB) family and WRKY family transcription factors. After SA pre-treatment, 697 genes and 124 proteins were differentially expressed. Pathway analysis showed similar enrichments in GSH, glyoxylate and dicarboxylate metabolism. Transcription factors were distributed in basic helix-loop-helix (bHLH), MYB, Tify and WRKY families. Quantitative real-time PCR results revealed that quinclorac stress induced the expression of glutathion reductase (GR) genes (, ), which was further pronounced by SA pre-treatment. Quinclorac stress further mediated the accumulation of acetaldehyde in rice, while SA enhanced the expression of and to accelerate the metabolism of herbicide quinclorac for the protection of rice. Correlation analysis between transcriptome and proteomics demonstrated that, under quinclorac stress, correlated proteins/genes were mainly involved in the inhibition of intermediate steps in the biosynthesis of chlorophyll. Other interesting proteins/genes and pathways regulated by herbicide quinclorac and modulated by SA pre-treatment were also discussed, based on the transcriptome and proteomics results.
为了揭示水杨酸（SA）对氯喹酸胁迫的缓解机制，本研究分别对经氯喹酸胁迫和经 SA 预处理的 ssp. 叶片样本进行了转录组和蛋白质组谱分析，以确定差异表达基因（DEGs）和蛋白（DEPs）。结果表明，氯喹酸胁迫改变了 2207 个 DEGs（1427 个上调，780 个下调）和 147 个 DEPs（98 个下调，49 个上调）的表达。这些基因和蛋白富集于谷胱甘肽（GSH）代谢、卟啉和叶绿素代谢、次生代谢物的生物合成、乙醛酸和二羧酸代谢等途径。它还影响了 AP2-乙烯反应元件结合蛋白（AP2-EREBP）家族、髓细胞瘤（MYB）家族和 WRKY 家族转录因子。经 SA 预处理后，有 697 个基因和 124 个蛋白差异表达。通路分析显示 GSH、乙醛酸和二羧酸代谢也存在类似的富集。转录因子分布在基本螺旋-环-螺旋（bHLH）、MYB、Tify 和 WRKY 家族中。实时定量 PCR 结果表明，氯喹酸胁迫诱导了谷胱甘肽还原酶（GR）基因（、）的表达，而 SA 预处理进一步增强了其表达。氯喹酸胁迫进一步介导了水稻中乙醛的积累，而 SA 则增强了和的表达，以加速除草剂氯喹酸的代谢，从而保护水稻。转录组和蛋白质组的相关性分析表明，在氯喹酸胁迫下，相关的蛋白/基因主要参与了叶绿素生物合成中间步骤的抑制。基于转录组和蛋白质组的结果，还讨论了其他由除草剂氯喹酸调节且经 SA 预处理调节的有趣蛋白/基因和途径。
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