Marshall M J, Nisbet N W, Green P M
Calcif Tissue Int. 1986 May;38(5):268-74. doi: 10.1007/BF02556605.
Osteoclastlike cells (OLCs) were recognized by their morphological, histochemical, and behavioral characteristics. These characteristics were initially observed in freshly isolated osteoclasts when neonatal rat bone cells were introduced into culture. Epithelioid cells proliferated during the first week to form a confluent layer. The cells' alkaline phosphatase content and ability to produce elevated levels of cyclic adenosine monophosphate in response to parathyroid hormone (PTH) led us to tentatively describe them as osteoblastlike cells. When a second addition of neonatal bone cells was made onto this confluent layer, OLCs were produced, their numbers increasing to a maximum 7 days later and dying out 3 weeks later. Mononuclear cells with similar characteristics to these multinuclear OLCs were observed and their production and disappearance closely paralleled that of the OLCs. Fusion of OLCs was observed. The conditions under which OLCs were produced are described.
破骨细胞样细胞（OLCs）通过其形态学、组织化学和行为特征得以识别。这些特征最初是在将新生大鼠骨细胞引入培养时，在刚分离出的破骨细胞中观察到的。上皮样细胞在第一周增殖形成汇合层。细胞的碱性磷酸酶含量以及对甲状旁腺激素（PTH）产生高水平环磷酸腺苷的能力，使我们初步将它们描述为成骨细胞样细胞。当在这个汇合层上再次添加新生骨细胞时，会产生OLCs，其数量在7天后增加到最大值，并在3周后消失。观察到了具有与这些多核OLCs相似特征的单核细胞，它们的产生和消失与OLCs密切平行。观察到了OLCs的融合。描述了产生OLCs的条件。
