Department of Analytical Chemistry, Regional Centre of Advanced Technologies and Materials, Faculty of Science, Palacký University in Olomouc, 17. listopadu 12, 77146 Olomouc, Czech Republic.
Department of Analytical Chemistry, Regional Centre of Advanced Technologies and Materials, Faculty of Science, Palacký University in Olomouc, 17. listopadu 12, 77146 Olomouc, Czech Republic.
Talanta. 2019 Jun 1;198:154-158. doi: 10.1016/j.talanta.2019.01.097. Epub 2019 Jan 29.
The aim of this study was to focus on the reduction of chiral selector concentration, sulfated-β-cyclodextrin, in an attempt decrease the running costs associated with separating cetirizine enantiomers by capillary electrophoresis. The decrease in the concentration of chiral selector was achieved by adding D-glucose to the background electrolyte, which consisted of sodium borate. Optimal separation of cetirizine enantiomers was obtained in the electrolyte containing 500 mmol L borate pH 9.5 with 1.0 mg mL sulfated-β-cyclodextrin, and 1000 mmol LD-glucose. This means a 15-fold reduction in the concentration of sulfated-β-cyclodextrin. The mechanism of the separation in this electrolyte was investigated using direct injection mass spectrometry. The electrolyte of borate, D-glucose, and sulfated-β-cyclodextrin forms a dual selector system, in which one selector is represented by the sulfated-β-cyclodextrin and the second selector is represented by the D-glucose-borate complexes.
本研究旨在关注手性选择剂（磺化-β-环糊精）浓度的降低，尝试通过毛细管电泳分离西替利嗪对映体来降低与运行成本相关的费用。通过在硼酸钠背景电解质中添加 D-葡萄糖，实现了手性选择剂浓度的降低。在含有 500mmol/L 硼酸盐 pH 9.5 的电解质中，加入 1.0mg/mL 的磺化-β-环糊精和 1000mmol/L 的 D-葡萄糖，可获得最佳的西替利嗪对映体分离效果。这意味着磺化-β-环糊精的浓度降低了 15 倍。使用直接注射质谱法研究了该电解质中的分离机制。硼酸盐、D-葡萄糖和磺化-β-环糊精的电解质形成了双选择剂体系，其中一个选择剂由磺化-β-环糊精表示，第二个选择剂由 D-葡萄糖-硼酸盐配合物表示。
