Institute of Nano Biomedicine and Engineering, Department of Instrument Science & Engineering, School of Electronic Information and Electrical Engineering, Shanghai Jiao Tong University, 800 Dongchuan RD, Shanghai 200240, China; Shanghai Engineering Research Center for Intelligent Instrument for Diagnosis and Therapy, 800 Dongchuan RD, Shanghai 200240, China.
Institute of Nano Biomedicine and Engineering, Department of Instrument Science & Engineering, School of Electronic Information and Electrical Engineering, Shanghai Jiao Tong University, 800 Dongchuan RD, Shanghai 200240, China; Shanghai Engineering Research Center for Intelligent Instrument for Diagnosis and Therapy, 800 Dongchuan RD, Shanghai 200240, China.
Talanta. 2019 Jun 1;198:45-54. doi: 10.1016/j.talanta.2019.01.085. Epub 2019 Jan 25.
The rapid identification of living cancer cells is highly crucial for cancer diagnosis, prognosis, and treatment monitoring. However, it is a great challenge to develop an effective way for rapid identification and imaging of cancer cells in a living state. Moreover, synthesis of monodisperse nanoparticles (NPs) with high sensitive surface-enhanced Raman scattering (SERS) activity is also a tough work. Herein, we creatively reported a convenient method to synthesize the novel NPs as the substrate of SERS sensors, which possessed a gold nanobipyramid core and silver nanorod shell. These gold nanobipyramid core and silver nanorod shell NPs (Au NBP@Ag NRs) were further modified with 4-mercaptobenzoicacid (4-MBA, Raman reporter molecule) and then conjugated with reduced bovine serum albumin (rBSA) and folic acid (FA) on their surfaces, to finally acquire Au NBP@Ag NR-MBA-rBSA-FA nanoprobes. In this system, With the enhancement factor (EF) of Au NBP@Ag NRs was about 4 × 10, it could significantly enhance Raman signal for Raman reporter molecules, and 4-MBA molecules performed high SERS signals based on their structures; the nanoprobes have favorable specificity and biocompatibility owing to the modification of rBSA which effectively avoided the nonspecific attachment of non-targeted cells. Moreover, the obtained SERS nanoprobes have excellent sensitivity for gastric cancer cells (MGC-803 cells) due to the conjugation of folic acid. Thus, the finally obtained Au NBP@Ag NR-MBA-rBSA-FA nanoprobes possess excellent detection efficiency for living MGC-803 cells. Therefore, our synthesized nanoprobes exhibit ultrasensitive SERS-activity, excellent specificity and superior cancer cells targeting ability, which could be applied for rapid identification and Raman imaging of living cancer cells via the SERS signal detection of the nanoprobes.
快速识别活癌细胞对于癌症的诊断、预后和治疗监测至关重要。然而，开发一种有效的方法来快速识别和成像活癌细胞仍然是一个巨大的挑战。此外，合成具有高灵敏表面增强拉曼散射（SERS）活性的单分散纳米粒子（NPs）也是一项艰巨的工作。在此，我们创造性地报道了一种简便的方法来合成具有金纳米双锥核和银纳米棒壳的新型 NPs 作为 SERS 传感器的基底。这些金纳米双锥核和银纳米棒壳 NPs（Au NBP@Ag NRs）进一步用 4-巯基苯甲酸（4-MBA，拉曼报告分子）修饰，然后在其表面与还原牛血清白蛋白（rBSA）和叶酸（FA）偶联，最终获得 Au NBP@Ag NR-MBA-rBSA-FA 纳米探针。在该系统中，由于 Au NBP@Ag NRs 的增强因子（EF）约为 4×10，因此可以显著增强拉曼报告分子的拉曼信号，并且由于其结构，4-MBA 分子表现出高 SERS 信号；由于 rBSA 的修饰，纳米探针具有良好的特异性和生物相容性，有效地避免了非靶向细胞的非特异性附着。此外，由于叶酸的偶联，获得的 SERS 纳米探针对胃癌细胞（MGC-803 细胞）具有优异的灵敏度。因此，最终获得的 Au NBP@Ag NR-MBA-rBSA-FA 纳米探针对活 MGC-803 细胞具有优异的检测效率。因此，我们合成的纳米探针具有超灵敏的 SERS 活性、优异的特异性和优异的癌细胞靶向能力，可通过纳米探针的 SERS 信号检测用于快速识别和活癌细胞的拉曼成像。
