Department of Chemistry and Institutes of Biomedical Sciences, Fudan University, Shanghai 200433, China.
Department of Chemistry and Institutes of Biomedical Sciences, Fudan University, Shanghai 200433, China.
Talanta. 2017 Sep 1;172:176-181. doi: 10.1016/j.talanta.2017.05.033. Epub 2017 May 17.
A novel, high-sensitivity, biocompatible SERS tag with core-shell structure based on gold nanoparticles containing alkynyl molecule core -silver nanoparticle satellites shell was fabricated for the first time to be used for live cancer cells Surface enhanced Raman scattering (SERS) imaging. (E)-2-((4-(phenylethynyl)benzylidene) amino) ethanethiol (PBAT) synthesized facilely in our lab is the Raman-silence region reporter which is advantage for bioorthogonal SERS cell imaging. In order to enhance the intensity of the Raman tags for live cancer cell imaging, a series of news measures have been adopted. Firstly, reporter molecules of the PBAT were added twice, which is embedded in the gold core with the reduction of tetrachloroaurate and then PBAT is conjugated again on disperse gold nanoparticles (PBAT-Au). Furthermore, numerous Ag nanoparticles self-assembly were densely arranged around PBAT-Au core surface (PBAT- Au@Ag), just like a circle of satellites cluster, which produce obvious "hot spots" effects enhancing the signal of the Raman tags enormously. Finally, Bovine serum albumin (BSA) and polydopamine (PDA) coated on the PBAT- Au@Ag successively, defined as (PBAT-Au@Ag@BSA@PDA), which make as-synthesized nanocomposites own features of bio-compatibility and facilitates antibody modification. Compared with Au@PBAT@PDA, PBAT-Au@Ag@BSA@PDA with core-shell satellites structure showed 10-fold increase in the Raman signals intensity. Moreover, PBAT-Au@Ag@BSA@PDA nanocomposites were successfully applied in the Raman imaging of human glioma cells (U251) by the recognition of the anti-epidermal growth factor receptor (EGFR). All experimental results demonstrated that the nanocomposites have high value and huge potential application in the live cancer cells imaging and biomedical diagnostics in the near future.
首次制备了一种新型的、高灵敏度、基于金纳米粒子的核壳结构的生物相容性 SERS 标签，该标签包含炔基分子核-银纳米粒子卫星壳，用于活癌细胞表面增强拉曼散射（SERS）成像。（E）-2-（（4-（苯乙炔基）亚苄基）氨基）乙硫醇（PBAT）是我们实验室合成的拉曼沉默区域报告分子，有利于生物正交 SERS 细胞成像。为了增强用于活癌细胞成像的 Raman 标签的强度，采取了一系列新措施。首先，添加了两次 PBAT 报告分子，它们被嵌入四氯金酸还原的金核中，然后再将 PBAT 再次连接到分散的金纳米粒子（PBAT-Au）上。此外，大量的 Ag 纳米粒子自组装密集排列在 PBAT-Au 核表面周围（PBAT-Au@Ag），就像一圈卫星簇一样，产生明显的“热点”效应，极大地增强了 Raman 标签的信号。最后，牛血清白蛋白（BSA）和聚多巴胺（PDA）分别涂覆在 PBAT-Au@Ag 上，定义为（PBAT-Au@Ag@BSA@PDA），使合成的纳米复合材料具有生物相容性的特点，并有利于抗体修饰。与 Au@PBAT@PDA 相比，具有核壳卫星结构的 PBAT-Au@Ag@BSA@PDA 的 Raman 信号强度增加了 10 倍。此外，PBAT-Au@Ag@BSA@PDA 纳米复合材料成功应用于人神经胶质瘤细胞（U251）的 Raman 成像，通过识别表皮生长因子受体（EGFR）。所有实验结果表明，该纳米复合材料在活癌细胞成像和生物医学诊断方面具有很高的价值和巨大的潜在应用前景。
