Boccadifuoco Giuseppe, Brunelli Brunella, Mori Elena, Agnusdei Mauro, Gianfaldoni Claudia, Giuliani Marzia Monica
GSK Vaccines, Siena, Italy.
Methods Mol Biol. 2019;1969:205-215. doi: 10.1007/978-1-4939-9202-7_14.
Meningococcal Antigen Typing System (MATS) is the combination of a sandwich ELISA (Enzyme Linked Immunosorbent Assay) developed to estimate the level of expression and immunoreactivity of the antigen components (fHbp, NHBA, and NadA) of the 4CMenB vaccine (Bexsero, GSK Vaccines) in circulating, serogroup B meningococcal (MenB) strains, with the molecular typing of PorA, the main antigenic component in the outer membrane vesicles (OMV). MATS has been proven to be a good surrogate of the accepted correlate of protection for meningococcus (hSBA), thus providing a quick, conservative and reproducible method to assess vaccine coverage. The method has been successfully transferred and standardized in several public health laboratories across Europe, North America, and Australia and used to screen thousands of isolates all over the world. Here we describe the sandwich ELISA method applied to assess the expression and cross-reactivity of fHbp, NHBA, and NadA in MenB isolates.
脑膜炎球菌抗原分型系统（MATS）是一种夹心酶联免疫吸附测定（ELISA）方法，用于评估4CMenB疫苗（Bexsero，葛兰素史克疫苗公司）的抗原成分（fHbp、NHBA和NadA）在循环的B群脑膜炎球菌（MenB）菌株中的表达水平和免疫反应性，并结合对外膜囊泡（OMV）中的主要抗原成分PorA进行分子分型。MATS已被证明是脑膜炎球菌公认的保护相关指标（hSBA）的良好替代指标，从而提供了一种快速、保守且可重复的方法来评估疫苗覆盖率。该方法已在欧洲、北美和澳大利亚的多个公共卫生实验室成功转移并标准化，并用于筛查全球数千株分离株。在此，我们描述了用于评估MenB分离株中fHbp、NHBA和NadA的表达及交叉反应性的夹心ELISA方法。
