Laboratoire Barla, 6 rue Barla, Nice, France.
Institut de médecine légale, 11 rue Humann, Strasbourg, France.
J Anal Toxicol. 2020 Jan 7;44(1):75-80. doi: 10.1093/jat/bkz011.
The authors aim to report a case of surreptitious administration of a synthetic cannabinoid (SC) and the subsequent toxicological investigations to be able to document accurately the case for submission at a hearing. A dealer gave surreptitiously a substance to two juvenile migrants who experienced shakings and faintness. The laboratory received a blood sample from each of the two victims, who, according to the investigators, were probably exposed to SGT-151, a SC, also known as CUMYL-PEGACLONE. Blood and urine specimens from the dealer, who claimed being a user of SGT-151 were received at the same time. To characterize the metabolites of SGT-151, the drug was incubated with a pool of human hepatic microsomes and the cofactors required to ensure the functioning of the main Phase I and Phase II enzymes. The incubation media were analyzed by liquid chromatography coupled to high-resolution mass spectrometry. The metabolites identified following transformation by hepatic microsomes were mostly N-dealkylated SGT-151, mono-hydroxylated SGT-151 and di-hydroxylated SGT-151. The presence of SGT-151 (5.4 ng/mL) and its metabolite, N-dealkyl SGT-151, was confirmed in the dealer's blood. Two metabolites of SGT-151 (OH-SGT-151, diOH-SGT-151) were detected in the dealer's urine. SGT-151 (~1 ng/mL) and its metabolite N-dealkyl SGT-151 were detected in the blood samples of the two victims.
作者旨在报告一起隐匿使用合成大麻素（SC）的案例，以便为提交听证会准确记录案件进行毒理学调查。一名毒贩偷偷将一种物质给了两名经历了摇晃和昏厥的未成年移民。实验室收到了来自两名受害者的血液样本，据调查人员称，他们可能接触到了 SGT-151，一种也称为 CUMYL-PEGACLONE 的 SC。同时收到了一名声称自己是 SGT-151 用户的毒贩的血液和尿液样本。为了表征 SGT-151 的代谢物，将药物与人类肝微粒体池和确保主要 I 期和 II 期酶正常运行所需的辅助因子一起孵育。通过液相色谱-高分辨率质谱联用分析孵育介质。经肝微粒体转化后鉴定出的代谢物主要为 SGT-151 的 N-去烷基化、单羟基化和二羟基化产物。在毒贩的血液中确认存在 SGT-151（5.4ng/mL）及其代谢物 N-去烷基 SGT-151。在毒贩的尿液中检测到 SGT-151 的两种代谢物（OH-SGT-151、diOH-SGT-151）。在两名受害者的血液样本中检测到 SGT-151（~1ng/mL）及其代谢物 N-去烷基 SGT-151。
