Jiao Tai-Feng, Li Ya-Qing, Kang Gang, Chen Shen-Shu, Cheng Liang-Hong
Guangdong Nantian Institute of Forensic Science, Shenzhen 518003, Guangdong Province, China.
Fa Yi Xue Za Zhi. 2022 Oct 25;38(5):595-600. doi: 10.12116/j.issn.1004-5619.2022.320701.
To establish a combined high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) and gas chromatography-mass spectrometry (GC-MS) method to detect the synthetic cannabinoid CUMYL-PEGACLONE in e-cigarette oil and hair.
HPLC-MS/MS and GC-MS were used to establish the detection method of CUMYL-PEGACLONE, and the hair of drug-involved persons and the seized e-cigarette oil were detected.
The main mass spectrometry characteristic ions of CUMYL-PEGACLONE measured by GC-MS were 91, 179, 197, 254 and 372. CUMYL-PEGACLONE had a good linear relationship in the mass concentration range of 2-50 ng/mL, and the linear correlation coefficient () was greater than 0.99. The limit of detection (LOD) of CUMYL-PEGACLONE in hair was 0.01 ng/mg, and the limit of quantitation (LOQ) was 0.02 ng/mg. The LOD of CUMYL-PEGACLONE in e-cigarette oil was 1 ng/mg, and the LOQ was 2 ng/mg. The average recoveries of CUMYL-PEGACLONE under the attempt at high, intermediate and low levels in blank human hair and e-cigarette oil matrix were 98.2%-132.4% and 93.5%-110.6%, and the intraday and intraday precision were 1.2%-12.9% and 0.7%-2.9%. CUMYL-PEGACLONE was detected in the hair of 15 drug-involved persons. Except for 1 person who was lower than LOQ, the concentration of CUMYL-PEGACLONE in the hair of other 14 persons was 0.035-0.563 ng/mg. The mass fraction of CUMYL-PEGACLONE in 2 e-cigarette oil were 0.17% and 0.21%, respectively.
The established HPLC-MS/MS and GC-MS methods are applied to the detection of HPLC-MS/MS in drug-related cases, which provides strong evidence support for the handling authority to quickly investigate these cases, and also provides a reference for the identification of such substances in future.
建立一种结合高效液相色谱 - 串联质谱法（HPLC-MS/MS）和气相色谱 - 质谱法（GC-MS）检测电子烟油和毛发中合成大麻素CUMYL-PEGACLONE的方法。
采用HPLC-MS/MS和GC-MS建立CUMYL-PEGACLONE的检测方法，并对涉毒人员毛发和查获的电子烟油进行检测。
GC-MS测定CUMYL-PEGACLONE的主要质谱特征离子为91、179、197、254和372。CUMYL-PEGACLONE在质量浓度范围为2 - 50 ng/mL时具有良好的线性关系，线性相关系数（）大于0.99。CUMYL-PEGACLONE在毛发中的检测限（LOD）为0.01 ng/mg，定量限（LOQ）为0.02 ng/mg。CUMYL-PEGACLONE在电子烟油中的LOD为1 ng/mg，LOQ为2 ng/mg。CUMYL-PEGACLONE在空白人发和电子烟油基质中高、中、低水平加样的平均回收率分别为98.2% - 132.4%和93.5% - 110.6%，日内和日间精密度分别为1.2% - 12.9%和0.7% - 2.9%。在15名涉毒人员毛发中检测到CUMYL-PEGACLONE。除1人低于LOQ外，其他14人毛发中CUMYL-PEGACLONE的浓度为0.035 - 0.563 ng/mg。2份电子烟油中CUMYL-PEGACLONE的质量分数分别为0.17%和0.21%。
所建立的HPLC-MS/MS和GC-MS方法应用于涉毒案件中CUMYL-PEGACLONE的检测，为办案机关快速侦查此类案件提供了有力证据支持，也为今后此类物质的鉴定提供了参考。
