新型双光子荧光探针，具有高荧光量子产率，可用于跟踪生物体系中的脂滴。
Novel two-photon fluorescent probe with high fluorescence quantum yields for tracking lipid droplets in biological systems.
机构信息
Institute of Fluorescent Probes for Biological Imaging, School of Materials Science and Engineering, School of Chemistry and Chemical Engineering, University of Jinan, Shandong 250022, PR China.
Institute of Fluorescent Probes for Biological Imaging, School of Materials Science and Engineering, School of Chemistry and Chemical Engineering, University of Jinan, Shandong 250022, PR China.
出版信息
Spectrochim Acta A Mol Biomol Spectrosc. 2019 Jun 5;216:35-44. doi: 10.1016/j.saa.2019.02.106. Epub 2019 Feb 27.
Lipid droplets (LDs) played an important role in storing neutral lipids process. In this work, we developed a series of fluorescent dyes PIE1-PIE4 with high fluorescence quantum yields based on a single phenanthrenequinone imidazole fluorophore core. Among these compounds, we introduced diethyl aniline group on the phenanthrenequinone imidazole core to provide the compound PIE1. It is firstly found that PIE1 could real-color image and track lipid droplets (LDs) in living cells. However, we modified nitrile, imidazole and methyl group on the same fluorophore core to afford three dyes PIE2, PIE3 and PIE4, respectively. Although PIE2, PIE3 and PIE4 possessed high fluorescence quantum yields in various organic solvents, they could not real-color image and track LDs in living cells.
脂滴（LDs）在储存中性脂质的过程中起着重要作用。在这项工作中，我们基于单苯并[g,h]喹啉亚胺荧光团核心，开发了一系列具有高荧光量子产率的荧光染料 PIE1-PIE4。在这些化合物中，我们在苯并[g,h]喹啉亚胺核心上引入二乙苯胺基团，得到化合物 PIE1。我们首次发现 PIE1 可以在活细胞中真实显色并跟踪脂滴（LDs）。然而，我们在同一荧光团核心上修饰了腈、咪唑和甲基，分别得到三种染料 PIE2、PIE3 和 PIE4。尽管 PIE2、PIE3 和 PIE4 在各种有机溶剂中具有高荧光量子产率，但它们不能在活细胞中真实显色和跟踪 LDs。
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