Beijing National Laboratory for Molecular Sciences, State Key Laboratory of Rare Earth Materials Chemistry and Applications, College of Chemistry and Molecular Engineering, Peking University, Beijing, 100871, P.R. China.
Department of Physiology and Pathophysiology, School of Basic Medical Sciences, Peking University, Beijing, 100191, P.R. China.
Chem Asian J. 2017 Oct 5;12(19):2533-2538. doi: 10.1002/asia.201701010. Epub 2017 Sep 6.
Designing two-photon probes for precise labeling of lipid droplets (LDs) and monitoring LD dynamics in adipocytes is of great significance to understand LD homeostasis. We herein report that a luminescent metal complex LD-TPZn can specifically image LDs in adipose cells and tissue using one- or two-photon fluorescence microscopy. Importantly, LD-TPZn exhibited higher specificity to LDs than the commercial dyes Nile Red and Bodipy 493/503, probably due to different cellular uptake pathways, that is, clathrin-mediated endocytosis and non-selectively passive diffusion, respectively. More importantly, LD-TPZn can be applied as a two-photon LD probe to image adipose tissue, one of the most challengeable tissues for traditional one-photon fluorescence microscope imaging due to the strong light scattering. Most importantly, LD-TPZn can be used to monitor LD growth during adipogenesis of preadipocytes, which is highly desirable to unravel the relationship between LD homeostasis and metabolic diseases.
设计用于精确定位脂滴 (LDs) 并监测脂肪细胞中 LD 动力学的双光子探针对于了解 LD 动态平衡具有重要意义。本文报道了一种发光金属配合物 LD-TPZn，可使用单光子或双光子荧光显微镜特异性地对脂肪细胞和组织中的 LDs 进行成像。重要的是，LD-TPZn 对 LDs 的特异性高于商业染料尼罗红和 Bodipy 493/503，这可能是由于不同的细胞摄取途径，即网格蛋白介导的内吞作用和非选择性被动扩散。更重要的是，LD-TPZn 可用作双光子 LD 探针来对脂肪组织进行成像，由于脂肪组织的强散射，这是传统单光子荧光显微镜成像最具挑战性的组织之一。最重要的是，LD-TPZn 可用于监测前体脂肪细胞成脂过程中 LD 的生长，这对于揭示 LD 动态平衡与代谢疾病之间的关系非常重要。
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