Mortimer D, Curtis E F, Dravland J E
Fertil Steril. 1986 Jul;46(1):97-103. doi: 10.1016/s0015-0282(16)49465-3.
An improved procedure for human sperm capacitation based upon calcium deprivation using a modified Tyrode's medium is described. Replacement of CaCl2 by SrCl2 significantly increased the penetration of zona-free hamster eggs after 20 hours of sperm preincubation, compared with parallel sperm aliquots incubated in normal medium. The effect of an extra 4 hours' sperm preincubation produced no significant shift in fertilizing ability. Addition of 0.1 mM ethyleneglycol-bis-(beta-amino-ethyl ether)-N,N,N',N'- tetraacetic acid (EGTA) to the strontium-substituted medium resulted in a further significant increase in the penetration rate as well as a significant increase in polyspermy. The average increase in the penetration capacity (penetration rate X polyspermy) of the strontium/EGTA-preincubated spermatozoa was approximately tenfold, with no significant shift as a result of an extra 4 hours' preincubation.
本文描述了一种基于使用改良的台氏液进行钙剥夺的人类精子获能的改进方法。与在正常培养基中孵育的平行精子等分试样相比，用SrCl2替代CaCl2在精子预孵育20小时后显著增加了去透明带仓鼠卵的穿透率。额外4小时的精子预孵育对受精能力没有显著影响。向锶替代培养基中添加0.1 mM乙二醇双（β-氨基乙醚）-N，N，N'，N'-四乙酸（EGTA）导致穿透率进一步显著增加以及多精受精显著增加。经锶/EGTA预孵育的精子的穿透能力（穿透率×多精受精）平均增加约10倍，额外4小时的预孵育没有导致显著变化。
