Kikuchi T, Mizuno K
Albrecht Von Graefes Arch Klin Exp Ophthalmol. 1978 Jul 17;207(2):83-90. doi: 10.1007/BF00414305.
The role of lysosomal enzymes in ischemic retinal pigment epithelial cells (RPE) of albino rabbits was examined with the modified Gomori technique for acid phosphatase. Ischemia was produced by cutting the lateral posterior ciliary artery and short posterior ciliary arteries (PCA). Five days after PCA-cut RPE in the ischemic region were disorganized by increased enzymatic digestion. In RPE of the border between the ischemic and normal region a lot of fragmented outer segments were phagocytosed 24 h to 7 days after PCA-cut. At this time phagosomes appeared much more frequently than in the normal retina, showing several variations in their shape, localization, and histochemical reaction. However, after 10 and 14 days, RPE in the border had a tendency to reduce the phagocytic activity. A strong acid phosphatase activity was encountered in the phagosomes of macrophages, which was considered to be derived from RPE, and seemed to play the major role in scavenging destructed retinal elements.
采用改良的戈莫里酸性磷酸酶染色技术，对白化兔缺血视网膜色素上皮细胞（RPE）中溶酶体酶的作用进行了研究。通过切断睫状后外侧动脉和睫状后短动脉（PCA）制造缺血模型。切断PCA后5天，缺血区域的RPE因酶消化增加而紊乱。在缺血区与正常区边界的RPE中，切断PCA后24小时至7天，大量破碎的外节被吞噬。此时，吞噬体比正常视网膜中出现得更为频繁，其形状、定位和组织化学反应呈现出多种变化。然而，10天和14天后，边界处的RPE吞噬活性有降低的趋势。在巨噬细胞的吞噬体中发现了强烈的酸性磷酸酶活性，这被认为源自RPE，似乎在清除受损视网膜成分中起主要作用。
