Beijing Advanced Innovation Center for Food Nutrition and Human Health, China Agricultural University, Beijing, 100193, China.
Department of Animal Nutrition and Feed Science, State Key Laboratory of Animal Nutrition, China Agricultural University, Beijing, 100193, China.
Amino Acids. 2019 May;51(5):805-811. doi: 10.1007/s00726-019-02717-2. Epub 2019 Mar 16.
We recently reported that dietary supplementation with L-proline (proline) during gestation improved embryonic survival in C57BL/6J mice. The objective of the present study was to test the hypothesis that the effect of maternal proline supplementation on embryonic survival can be carried forward to the first generation female offspring. In the F0 generation, pregnant dams were fed a purified diet supplemented with 0 (control) or 5 g proline/kg diet. The F1 female adult offsprings were bred to fertile males. Fetal survival at embryonic day (E)12.5 and reproductive outcomes at term birth were recorded. The concentrations of amino acids, ammonia, and urea in plasma and amniotic fluid, as well as concentrations of polyamines in placental tissues and amniotic fluid at E12.5 were determined. Results showed that the F1 generation female offspring from proline-supplemented dams had higher (P < 0.05) concentrations of glutamate and taurine in plasma; of putrescine and spermidine in placental tissues; and of glycine, taurine, and spermidine in amniotic fluid at E12.5, as compared with F1 generation female offsprings from dams without proline supplementation. Concentration of proline in the plasma of offspring mice from proline-supplemented dams were lower (P < 0.05), as compared with the control group. No differences in fetal survival, reproductive outcomes, or concentrations of ammonia and urea in plasma and amniotic fluid were observed between the two groups of F1 female offspring. Collectively, our results indicate that the benefits of maternal proline supplementation during gestation on improving embryonic survival and fetal growth in F0 females are not transmitted to their F1 generation females.
我们最近报道称，妊娠期间补充 L-脯氨酸（脯氨酸）可提高 C57BL/6J 小鼠的胚胎存活率。本研究的目的是检验以下假设，即母体脯氨酸补充对胚胎存活率的影响可以传递给第一代雌性后代。在 F0 代中，怀孕的母鼠喂食添加 0（对照）或 5 g 脯氨酸/kg 饮食的纯化饮食。F1 代雌性成年后代与可育雄性交配。记录胚胎第 12.5 天（E）的胎儿存活率和足月出生的生殖结果。测定血浆和羊水的氨基酸、氨和尿素浓度，以及 E12.5 时胎盘组织和羊水的多胺浓度。结果表明，来自脯氨酸补充的母鼠的 F1 代雌性后代的血浆中谷氨酸和牛磺酸浓度较高（P < 0.05）；胎盘组织中腐胺和亚精胺浓度较高；E12.5 时羊水的甘氨酸、牛磺酸和亚精胺浓度较高，与来自未补充脯氨酸的母鼠的 F1 代雌性后代相比。脯氨酸补充组后代小鼠血浆中脯氨酸的浓度较低（P < 0.05），与对照组相比。两组 F1 代雌性后代之间的胎儿存活率、生殖结果或血浆和羊水的氨和尿素浓度均无差异。总之，我们的研究结果表明，母体妊娠期间补充脯氨酸对改善 F0 代雌性胚胎存活率和胎儿生长的益处不能传递给其 F1 代雌性。
