Sunny Armando, Duarte-deJesus Luis, Aguilera-Hernández Arlene, Ramírez-Corona Fabiola, Suárez-Atilano Marco, Percino-Daniel Ruth, Manjarrez Javier, Monroy-Vilchis Octavio, González-Fernández Andrea
Centro de Investigación en Ciencias Biológicas Aplicadas, Universidad Autónoma del Estado de México, Instituto Literario #100, Colonia Centro, 50000, Toluca, Mexico State, Mexico.
Taller de Sistemática y Biogeografía, Departamento de Biología Evolutiva, Facultad de Ciencias, Universidad Nacional Autónoma de México, Ciudad Universitaria, 04510, Mexico City, Mexico.
Genetica. 2019 Apr;147(2):149-164. doi: 10.1007/s10709-019-00058-2. Epub 2019 Mar 16.
Land use changes are threatening the maintenance of biodiversity. Genetic diversity is one of the main indicators of biological diversity and is highly important as it shapes the capability of populations to respond to environmental changes. We studied eleven populations of Pseudoeurycea robertsi, a micro-endemic and critically endangered species from the Nevado de Toluca Volcano, a mountain that is part of the Trans-Mexican Volcanic Belt, Mexico. We sequenced the mitochondrial cytochrome b gene from 71 individuals and genotyped 9 microsatellites from 150 individuals. Our results based on the cytochrome b showed two divergent lineages, with moderate levels of genetic diversity and a recently historical demographic expansion. Microsatellite-based results indicated low levels of heterozygosity for all populations and few alleles per locus, as compared with other mole salamander species. We identified two genetically differentiated subpopulations with a significant level of genetic structure. These results provide fundamental data for the development of management plans and conservation efforts for this critically endangered species.
土地利用变化正威胁着生物多样性的维持。遗传多样性是生物多样性的主要指标之一，非常重要，因为它塑造了种群应对环境变化的能力。我们研究了罗伯茨假螈的11个种群，这是一种来自墨西哥托卢卡火山的微型特有且极度濒危物种，该火山是墨西哥跨火山带的一部分。我们对71个个体的线粒体细胞色素b基因进行了测序，并对150个个体的9个微卫星进行了基因分型。基于细胞色素b的研究结果显示出两个不同的谱系，具有中等水平的遗传多样性和近期的历史种群扩张。基于微卫星的结果表明，与其他鼹钝口螈物种相比，所有种群的杂合度水平较低，每个位点的等位基因较少。我们识别出两个具有显著遗传结构水平的遗传分化亚种群。这些结果为制定该极度濒危物种的管理计划和保护措施提供了基础数据。
