Nibbering P H, Marijnen J G, Raap A K, Leijh P C, van Furth R
Histochemistry. 1986;84(4-6):538-43. doi: 10.1007/BF00482988.
The nitrocellulose binding assay was used for quantitative studies on the cytochemical reactions for the three enzymes most frequently used in immunocytochemistry. The results show a linear relationship between the amount of enzyme immobilized on nitrocellulose and the amount of the enzyme reaction product. The similar course of the formation of the reaction product after DAB/H2O2 staining for peroxidase immobilized on nitrocellulose and for immunoperoxidase labeled cells indicates a linear relationship between the amount of enzyme-coupled antibodies bound to cells and the amount of enzyme reaction product. Furthermore, a mild acid treatment for the abolition of endogeneous peroxidase activity in tissues and cells applicable to immunoperoxidase staining procedures is proposed.
硝酸纤维素结合试验用于对免疫细胞化学中最常用的三种酶的细胞化学反应进行定量研究。结果表明，固定在硝酸纤维素上的酶量与酶反应产物量之间呈线性关系。硝酸纤维素上固定的过氧化物酶经DAB/H₂O₂染色后以及免疫过氧化物酶标记细胞后反应产物形成的相似过程表明，与细胞结合的酶偶联抗体量与酶反应产物量之间呈线性关系。此外，还提出了一种适用于免疫过氧化物酶染色程序的用于消除组织和细胞中内源性过氧化物酶活性的温和酸处理方法。
