基于 MoS 纳米片的 pH 响应型三联体 DNA 器件
pH-Operated Triplex DNA Device on MoS Nanosheets.
机构信息
Shanghai Key Laboratory of Green Chemistry and Chemical Processes, School of Chemistry and Molecular Engineering , East China Normal University , 500 Dongchuan Road , Shanghai 200241 , P. R. China.
Institutes of Biomedical Sciences and Zhongshan Hospital , Fudan University , Shanghai 200032 , P. R. China.
出版信息
Langmuir. 2019 Apr 9;35(14):5050-5053. doi: 10.1021/acs.langmuir.8b04272. Epub 2019 Mar 25.
We report a triplex-based DNA device coupled with molybdenum disulfide (MoS) nanosheets for use as a pH-sensing platform. The device transitions from a duplex state at pH 8 to a triplex state at pH 5. The interaction of the device with MoS nanosheets in the two states is read out as a fluorescence signal from a pH-insensitive dye attached to the device. We characterized the operation of the DNA device on MoS nanosheets, analyzed the pH response, and tested the reversibility of the system. Our strategy can lead to the creation of a suite of biosensors where the sensing element is a triplex DNA device and the signal response is modulated by inorganic nanomaterials.
我们报告了一种基于三联体的 DNA 器件，该器件与二硫化钼 (MoS) 纳米片偶联，用作 pH 传感平台。该器件在 pH 8 时从双链状态转变为 pH 5 时的三链状态。在两种状态下，该器件与 MoS 纳米片的相互作用被读出为附着在器件上的对 pH 不敏感染料的荧光信号。我们在 MoS 纳米片上表征了 DNA 器件的工作原理，分析了 pH 响应，并测试了该系统的可逆性。我们的策略可以导致一系列生物传感器的创建，其中传感元件是三联体 DNA 器件，信号响应由无机纳米材料调制。
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