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哈氏弧菌和费氏弧菌的荧光素酶在丝状蓝细菌中的表达

Expression of luciferases from Vibrio harveyi and Vibrio fischeri in filamentous cyanobacteria.

作者信息

Schmetterer G, Wolk C P, Elhai J

出版信息

J Bacteriol. 1986 Jul;167(1):411-4. doi: 10.1128/jb.167.1.411-414.1986.

DOI:10.1128/jb.167.1.411-414.1986
PMID:3087964
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC212896/
Abstract

Shuttle vectors that had previously been shown to replicate both in Escherichia coli and in strains of Anabaena spp. were used to transfer the lux genes from Vibrio harveyi and Vibrio fischeri into Anabaena spp. The level of expression of luciferase in the cyanobacteria (up to 7,000 quanta cell-1 s-1) makes these genes good candidates for use as promoter probes during the differentiation of certain cells in a filament into heterocysts.

摘要

先前已证明能在大肠杆菌和鱼腥藻属菌株中复制的穿梭载体，被用于将哈维氏弧菌和费氏弧菌的荧光素酶基因转移到鱼腥藻属中。蓝细菌中荧光素酶的表达水平（高达7000光量子·细胞⁻¹·秒⁻¹）使得这些基因成为丝状藻丝中某些细胞分化为异形胞过程中用作启动子探针的良好候选基因。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/be3f/212896/dca24b248fc2/jbacter00206-0423-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/be3f/212896/dca24b248fc2/jbacter00206-0423-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/be3f/212896/dca24b248fc2/jbacter00206-0423-a.jpg

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本文引用的文献

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Isolation and sequence of the gene for the large subunit of ribulose-1,5-bisphosphate carboxylase from the cyanobacterium Anabaena 7120.从蓝藻鱼腥藻 7120 中分离并测定核酮糖-1,5-二磷酸羧化酶大亚基的基因序列。
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Developmental regulation and spatial pattern of expression of the structural genes for nitrogenase in the cyanobacterium Anabaena.蓝藻鱼腥藻中固氮酶结构基因的表达发育调控及空间模式
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Characterization of cryptic plasmids from marine cyanobacteria and construction of a hybrid plasmid potentially capable of transformation of marine cyanobacterium, Synechococcus sp., and its transformation.海洋蓝藻中隐蔽质粒的特性分析以及构建一种可能能够转化海洋蓝藻聚球藻属（Synechococcus sp.）的杂种质粒及其转化
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Identification and characterization of hetA, a gene that acts early in the process of morphological differentiation of heterocysts.异形胞形态分化过程早期起作用的基因hetA的鉴定与特性分析。
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