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哈氏弧菌和费氏弧菌的荧光素酶在丝状蓝细菌中的表达

Expression of luciferases from Vibrio harveyi and Vibrio fischeri in filamentous cyanobacteria.

作者信息

Schmetterer G, Wolk C P, Elhai J

出版信息

J Bacteriol. 1986 Jul;167(1):411-4. doi: 10.1128/jb.167.1.411-414.1986.

Abstract

Shuttle vectors that had previously been shown to replicate both in Escherichia coli and in strains of Anabaena spp. were used to transfer the lux genes from Vibrio harveyi and Vibrio fischeri into Anabaena spp. The level of expression of luciferase in the cyanobacteria (up to 7,000 quanta cell-1 s-1) makes these genes good candidates for use as promoter probes during the differentiation of certain cells in a filament into heterocysts.

摘要

先前已证明能在大肠杆菌和鱼腥藻属菌株中复制的穿梭载体,被用于将哈维氏弧菌和费氏弧菌的荧光素酶基因转移到鱼腥藻属中。蓝细菌中荧光素酶的表达水平(高达7000光量子·细胞⁻¹·秒⁻¹)使得这些基因成为丝状藻丝中某些细胞分化为异形胞过程中用作启动子探针的良好候选基因。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/be3f/212896/dca24b248fc2/jbacter00206-0423-a.jpg

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