Matsunaga T, Takeyama H, Nakamura N
Department of Biotechnology, Tokyo University of Agriculture and Technology, Japan.
Appl Biochem Biotechnol. 1990 Spring-Summer;24-25:151-60. doi: 10.1007/BF02920241.
Among forty strains of marine cyanobacteria isolated in our laboratory, five strains had 1-3 different plasmids. The unicellular marine cyanobacterium, Synechococcus sp. NKBG 042902, contains at least three plasmids (pSY09, pSY10, and pSY11). However, these plasmids are cryptic. Therefore, a hybrid plasmid pUSY02 containing the 1.4-kb HindIII fragment of pSY11 and Escherichia coli plasmid pUC18 was constructed. The plasmid pUSY02 transformed both marine Synechococcus sp. NKBG042902-YG1116, which is a cured strain, and fresh water Anacystis nidulans R2 by dark incubation or Ca2+ treatment. However, the plasmid pSG111 constructed from the plasmid DNA of A. nidulans R2 failed to transform marine Synechococcus sp. Electroporation was also applicable to transformation of marine Synechococcus sp. and fresh water A. nidulans R2. The plasmid pUSY02 was rapidly introduced into marine Synechococcus sp.
在我们实验室分离出的40株海洋蓝细菌中，有5株含有1至3种不同的质粒。单细胞海洋蓝细菌聚球藻属NKBG 042902至少含有三种质粒（pSY09、pSY10和pSY11）。然而，这些质粒是隐蔽型的。因此，构建了一种含有pSY11的1.4kb HindIII片段和大肠杆菌质粒pUC18的杂交质粒pUSY02。质粒pUSY02通过暗培养或Ca2+处理转化了海洋聚球藻属NKBG042902 - YG1116（一种治愈菌株）和淡水集胞藻6803 R2。然而，由集胞藻6803 R2的质粒DNA构建的质粒pSG111未能转化海洋聚球藻属。电穿孔也适用于海洋聚球藻属和淡水集胞藻6803 R2的转化。质粒pUSY02被快速导入海洋聚球藻属。
