Centro de Investigaciones y Transferencia de Villa María (CIT-VM), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Universidad Nacional de Villa María (UNVM), Villa María, Argentina.
Centro de Investigaciones y Transferencia de Villa María (CIT-VM), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Universidad Nacional de Villa María (UNVM), Villa María, Argentina; Instituto Académico Pedagógico de Ciencias Básicas y Aplicadas (IAPCByA), Universidad Nacional de Villa María (UNVM), Villa María, Argentina.
Carbohydr Polym. 2019 Jun 1;213:1-9. doi: 10.1016/j.carbpol.2019.02.016. Epub 2019 Feb 21.
Staphylococcus is the most commonly isolated genus from animals with intramammary infections, and mastitis is the most prevalent disease that affects dairy cows in many countries. These pathogens can live in biofilms, a self-produced matrix, which allow them evade the innate immune system and the antibiotic therapy, thereby producing persistent infections. The aim of this study was to explore the antimicrobial potential of chitosan nanoparticles (Ch-NPs) obtained by the reverse micellar method. We found that the nanoformulation developed presents antimicrobial activity against mastitis pathogens in a dose-dependent manner. Moreover, different experiments corroborated that the antimicrobial effectiveness of Ch-NP was greater than that shown by the native polymer used in the preparation of these nanocomposites. Ch-NPs caused membrane damage to bacterial cells and inhibited bacterial biofilm formation, without affecting the viability of bovine cells. These findings show the great potential of Ch-NPs as therapeutic agent for bovine mastitis treatment.
葡萄球菌属是动物中最常分离到的与乳腺炎有关的属，乳腺炎是许多国家奶牛最常见的疾病。这些病原体可以生活在生物膜中，这是一种自身产生的基质，可以使它们逃避先天免疫系统和抗生素治疗，从而产生持续性感染。本研究旨在探索反胶束法获得的壳聚糖纳米粒子（Ch-NPs）的抗菌潜力。我们发现，所开发的纳米制剂对乳腺炎病原体具有剂量依赖性的抗菌活性。此外，不同的实验证实，Ch-NP 的抗菌效果大于用于制备这些纳米复合材料的天然聚合物的抗菌效果。Ch-NPs 导致细菌细胞的膜损伤并抑制细菌生物膜的形成，而不影响牛细胞的活力。这些发现表明 Ch-NPs 作为治疗牛乳腺炎的治疗剂具有巨大的潜力。
