Crook S M, Treml S B, Collins M L
J Bacteriol. 1986 Jul;167(1):89-95. doi: 10.1128/jb.167.1.89-95.1986.
An immunocytochemical ultrastructural study of Rhodospirillum rubrum cultured under semiaerobic conditions was conducted to correlate the localization of functional components with membrane formation. R. rubrum is a facultatively phototrophic organism. Under reduced oxygen, this bacterium forms an intracytoplasmic chromatophore membrane that is the site of the photosynthetic apparatus. Immunogold techniques were used to localize intracellular protein antigens associated with the photosynthetic apparatus. Antibody, demonstrated by immunoblotting to be specific for the reaction center and light-harvesting photochemical components, was conjugated to colloidal gold particles and used for direct immunolabeling of fixed, sectioned specimens. Membrane invaginations appeared by 4 h after transition to induction conditions, and mature chromatophore membrane was abundant by 22 h. The occurrence of chromatophore membrane was correlated with bacteriochlorophyll a content and the density of the immunolabel. In uninduced (aerobic) cells and those obtained from cultures 0.5 h posttransition, the immunogold preferentially labeled the peripheral area of the cell. In contrast, in cells obtained after 22 h of induction, the central region of the cell was preferentially immunolabeled. These findings provided immunocytochemical evidence supporting the hypothesis that the chromatophore membrane is formed by invagination of the cytoplasmic membrane.
对在半好氧条件下培养的深红红螺菌进行了免疫细胞化学超微结构研究，以将功能成分的定位与膜形成相关联。深红红螺菌是一种兼性光合营养生物。在低氧条件下，这种细菌形成胞质内的载色体膜，该膜是光合装置的所在地。免疫金技术用于定位与光合装置相关的细胞内蛋白质抗原。通过免疫印迹证明对反应中心和捕光光化学成分具有特异性的抗体与胶体金颗粒偶联，并用于对固定的切片标本进行直接免疫标记。在转变至诱导条件后4小时出现膜内陷，到22小时时成熟的载色体膜丰富。载色体膜的出现与细菌叶绿素a含量和免疫标记密度相关。在未诱导（好氧）的细胞以及转变后0.5小时培养物中获得的细胞中，免疫金优先标记细胞的周边区域。相反，在诱导22小时后获得的细胞中，细胞的中央区域优先被免疫标记。这些发现提供了免疫细胞化学证据，支持了载色体膜是由细胞质膜内陷形成的假说。
