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通过免疫金标记检测两株甲基营养型细菌中甲醇脱氢酶的定位。

Localization of methanol dehydrogenase in two strains of methylotrophic bacteria detected by immunogold labeling.

作者信息

Fassel T A, Buchholz L A, Collins M L, Remsen C C

机构信息

Department of Biological Sciences, University of Wisconsin-Milwaukee 53201.

出版信息

Appl Environ Microbiol. 1992 Jul;58(7):2302-7. doi: 10.1128/aem.58.7.2302-2307.1992.

DOI:10.1128/aem.58.7.2302-2307.1992
PMID:1365400
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC195772/
Abstract

Antibodies to methanol dehydrogenase purified from Methylobacterium sp. strain AM1 and Methylomonas sp. strain A4 were raised. The antibody preparations were used in indirect immunogold labeling studies. With this approach, methanol dehydrogenase was found to be preferentially localized to the periplasmic region of the methylotroph Methylobacterium sp. strain AM1 and to the intracytoplasmic membrane of the methanotroph Methylomonas sp. strain A4. Antibody cross-reactivity to other methylotrophic bacteria was detected.

摘要

制备了针对从甲基杆菌属菌株AM1和甲基单胞菌属菌株A4中纯化得到的甲醇脱氢酶的抗体。这些抗体制剂用于间接免疫金标记研究。通过这种方法，发现甲醇脱氢酶优先定位于甲基营养菌甲基杆菌属菌株AM1的周质区域以及甲烷营养菌甲基单胞菌属菌株A4的胞内膜。检测到抗体与其他甲基营养菌的交叉反应性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7f03/195772/faee7fddcdf3/aem00048-0211-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7f03/195772/a6248cf91cb5/aem00048-0209-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7f03/195772/467a3fb42db0/aem00048-0210-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7f03/195772/faee7fddcdf3/aem00048-0211-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7f03/195772/a6248cf91cb5/aem00048-0209-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7f03/195772/467a3fb42db0/aem00048-0210-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7f03/195772/faee7fddcdf3/aem00048-0211-a.jpg

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