Institute of Oceanology, Polish Academy of Sciences, Sopot, Poland.
Institute of Oceanology, Polish Academy of Sciences, Sopot, Poland.
Anim Reprod Sci. 2019 May;204:10-21. doi: 10.1016/j.anireprosci.2019.02.014. Epub 2019 Mar 2.
The aim of this study was to determine changes in concentrations of melatonin (Mel) and thyroxine (T) in plasma, and 17β-estradiol (E) and 11-ketotestosterone (11-KT) in plasma and gonads of female and male round gobies (Neogobius melanostomus) from the Southern Baltic Sea in four phases of the reproductive cycle classified as pre-spawning, spawning, late spawning and non-spawning periods. The concentrations of Mel, T and E were determined by radioimmunoassay (RIA) whereas 11-KT was quantified using an enzyme immunoassay (EIA). The maturity stage of gonads was determined using histological analyses. The pattern of changes in Mel concentrations of females and males was similar with the greatest concentrations in the spawning and non-spawning phases. In both sexes, there was a similar tendency of change in concentrations of T and E with the increase being in the pre-spawning and non-spawning phases. The greatest concentrations of 11-KT were observed in the plasma and gonads of males during the spawning phase. In females, there were no changes in 11-KT concentrations either in plasma or gonads during all phases where quantifications occurred. This is the first study for determination of the pattern of changes in Mel and T concentrations as well as gonadal steroids E and 11-KT, supported by histological analysis of gonads, in batch spawning fish during the reproductive cycle.
本研究旨在确定波罗的海南部的圆鳍鱼（Neogobius melanostomus）在繁殖周期的四个阶段（产卵前、产卵期、后期产卵期和非产卵期）中，血浆中褪黑素（Mel）和甲状腺素（T）的浓度，以及血浆和性腺中 17β-雌二醇（E）和 11-酮睾酮（11-KT）的浓度变化。Mel、T 和 E 的浓度通过放射免疫分析（RIA）测定，而 11-KT 通过酶免疫分析（EIA）定量。性腺的成熟阶段通过组织学分析确定。雌性和雄性的 Mel 浓度变化模式相似，在产卵期和非产卵期浓度最高。在两性中，T 和 E 的浓度变化趋势相似，在产卵前和非产卵期增加。在产卵期，雄性血浆和性腺中 11-KT 的浓度最高。在所有发生定量的阶段，雌性血浆和性腺中 11-KT 的浓度都没有变化。这是首次在繁殖周期中批量产卵鱼类的组织学分析的基础上，确定 Mel 和 T 浓度以及性腺类固醇 E 和 11-KT 的变化模式的研究。
