Division of General Surgery, The Second Affiliated Hospital of Anhui Medical University, Hefei 230601, China.
Division of General Surgery, The First Affiliated Hospital of Anhui Medical University, Hefei 230022, China.
Hepatobiliary Pancreat Dis Int. 2019 Jun;18(3):266-272. doi: 10.1016/j.hbpd.2019.03.001. Epub 2019 Mar 5.
Clear tumor imaging is essential to the resection of hepatocellular carcinoma (HCC). This study aimed to create a novel biological probe to improve the HCC imaging.
Au nano-flower particles and CuInS-ZnS core-shell quantum dots were synthesized by hydrothermal method. Au was coated with porous SiO and combined with anti-AFP antibody. HCC cell line HepG2 was used to evaluate the targeting efficacy of the probe, while flow cytometry and MTT assay were used to detect the cytotoxicity and bio-compatibility of the probe. Probes were subcutaneously injected to nude mice to explore light intensity and tissue penetration.
The fluorescence stability of the probe was maintained 100% for 24 h, and the brightness value was 4 times stronger than that of the corresponding CuInS-ZnS quantum dot. In the targeting experiment, the labeled HepG2 emitted yellow fluorescence. In the cytotoxicity experiments, MTT and flow cytometry results showed that the bio-compatibility of the probe was fine, the inhibition rate of HepG2 cell with 60% Cu-QDs/Anti-AFP probe and Au-QDs/Anti-AFP probe solution for 48 h were significantly different (86.3%±7.0% vs. 4.9%±1.3%, t = 19.745, P<0.05), and the apoptosis rates were 83.3%±5.1% vs. 4.4%±0.8% (P<0.001). In the animal experiment, the luminescence of the novel probe can penetrate the abdominal tissues of a mouse, stronger than that of CuInS-ZnS quantum dot.
The Au@SiO@CuInS-ZnS/Anti-AFP probe can targetedly recognize and label HepG2 cells with good bio-compatibility and no toxicity, and the strong tissue penetrability of luminescence may be helpful to surgeons.
清晰的肿瘤成像对于肝细胞癌（HCC）的切除至关重要。本研究旨在创建一种新型生物探针以改善 HCC 成像。
通过水热法合成金纳米花颗粒和 CuInS-ZnS 核壳量子点。Au 被涂覆多孔 SiO 并与抗 AFP 抗体结合。使用 HCC 细胞系 HepG2 评估探针的靶向效果，同时使用流式细胞术和 MTT 测定法检测探针的细胞毒性和生物相容性。将探针皮下注射到裸鼠中以探索光强度和组织穿透性。
探针的荧光稳定性在 24 小时内保持 100％，亮度值比相应的 CuInS-ZnS 量子点强 4 倍。在靶向实验中，标记的 HepG2 发出黄色荧光。在细胞毒性实验中，MTT 和流式细胞术结果表明，探针的生物相容性良好，60％Cu-QDs/Anti-AFP 探针和 Au-QDs/Anti-AFP 探针溶液孵育 48 小时后 HepG2 细胞的抑制率有显著差异（86.3％±7.0％ vs. 4.9％±1.3％，t=19.745，P<0.05），凋亡率分别为 83.3％±5.1％和 4.4％±0.8％（P<0.001）。在动物实验中，新型探针的发光可以穿透小鼠的腹部组织，比 CuInS-ZnS 量子点更强。
Au@SiO@CuInS-ZnS/Anti-AFP 探针可以靶向识别和标记 HepG2 细胞，具有良好的生物相容性和无毒副作用，发光的强组织穿透性可能有助于外科医生。
