Maloney M S
J Cell Physiol. 1986 Jul;128(1):113-20. doi: 10.1002/jcp.1041280117.
Concanavalin A (Con A) has been shown to induce delays in oral regeneration in the ciliate Stentor coeruleus. Associated with the delayed oral regeneration is a shedding of the cell's extracellular pellicle with the loss of some pigment granules. It is shown that the delays in oral regeneration are not the result of the pigment shedding. The delays are localized in the earliest stages of oral regeneration prior to stage 4. The delays caused by Con A are completely reversible by the addition of 2 mg/ml alpha-D-methyl mannoside either at the time of Con A exposure or 5 min later. Con A clearly binds to the cell surface as shown by the binding of FITC-Con A and its reversal by alpha-methyl mannoside. Crosslinking of Con A receptor molecules may be responsible for the effects of Con A since succinyl Con A, which does not crosslink these receptors, has no effect on oral regeneration even at double the Con A concentration. Calcium ions are also implicated in the action of Con A because an excess of extracellular calcium (10 mM) completely eliminates the Con A delays when added simultaneously with Con A. Examination of the minimum extracellular calcium concentration required for this effect showed that 2 mM calcium can reverse most of the delays but that 5 mM is necessary to completely reverse the delays caused by Con A. If the addition of calcium is delayed for various times after Con A addition, the extracellular calcium is progressively less effective in reversing the Con A delays.
伴刀豆球蛋白A（Con A）已被证明会导致纤毛虫蓝喇叭虫的口部再生延迟。与口部再生延迟相关的是细胞外皮层的脱落以及一些色素颗粒的丧失。研究表明，口部再生延迟并非色素脱落的结果。这些延迟发生在口部再生的最早阶段，即4期之前。在接触Con A时或5分钟后添加2 mg/ml的α-D-甲基甘露糖苷，可使Con A引起的延迟完全逆转。如FITC-Con A的结合及其被α-甲基甘露糖苷逆转所示，Con A明显与细胞表面结合。Con A受体分子的交联可能是Con A产生作用的原因，因为不会交联这些受体的琥珀酰Con A即使在Con A浓度加倍时对口部再生也没有影响。钙离子也与Con A的作用有关，因为当与Con A同时添加过量的细胞外钙（10 mM）时，可完全消除Con A引起的延迟。对产生这种效果所需的最低细胞外钙浓度的研究表明，2 mM钙可逆转大部分延迟，但需要5 mM才能完全逆转Con A引起的延迟。如果在添加Con A后延迟不同时间添加钙，细胞外钙在逆转Con A延迟方面的效果会逐渐降低。
