小檗碱可减轻体外棕榈酸钠诱导的草鱼肝细胞脂质积累、氧化应激和凋亡。
Berberine attenuates sodium palmitate-induced lipid accumulation, oxidative stress and apoptosis in grass carp(Ctenopharyngodon idella)hepatocyte in vitro.
机构信息
Shanghai Key Laboratory for Veterinary and Biotechnology, School of Agriculture and Biology, Shanghai Jiao Tong University, No.800 Dongchuan Road, Shanghai, 200240, People's Republic of China.
Shanghai Key Laboratory for Veterinary and Biotechnology, School of Agriculture and Biology, Shanghai Jiao Tong University, No.800 Dongchuan Road, Shanghai, 200240, People's Republic of China.
出版信息
Fish Shellfish Immunol. 2019 May;88:518-527. doi: 10.1016/j.fsi.2019.02.055. Epub 2019 Mar 14.
The objective of this work was to investigate the effect of berberine (BBR) on the Cell viability, lipid accumulation, apoptosis, cytochrome c, caspase-9 and caspase-3 in lipid accumulation-hepatocytes induced by sodium palmitate in vitro. The lipid accumulation-hepatocytes (induced by 0.5 mM sodium palmitate for 24 h) were treated with 5 μM berberine for 12 h. Then, the Cell viability, intracellular triglyceride (TG) content, lipid peroxide (LPO), malonaldehyde (MDA) content, cytochrome c, caspase-9, caspase-3 and apoptosis were detected. Sodium palmitate decreased Cell viability and increased intracellular TG content, lipid droplet accumulation, LPO and MDA concentrations, caused caspase-3 and caspase-9 activation, then led to apoptosis accompanied by cytochrome c release from mitochondria into the cytoplasm. Beberine could improve intracellular lipid droplet accumulation and oxidative stress, while reduce apoptosis induced by sodium palmitate.
本研究旨在探讨小檗碱（BBR）对体外棕榈酸钠诱导的脂肪堆积肝细胞中细胞活力、脂滴积累、细胞凋亡、细胞色素 c、半胱氨酸天冬氨酸蛋白酶-9（caspase-9）和半胱氨酸天冬氨酸蛋白酶-3（caspase-3）的影响。用 0.5mM 棕榈酸钠处理细胞 24h 诱导脂肪堆积肝细胞，然后用 5μM 小檗碱处理细胞 12h。检测细胞活力、细胞内三酰甘油（TG）含量、脂质过氧化物（LPO）、丙二醛（MDA）含量、细胞色素 c、caspase-9、caspase-3 和细胞凋亡。结果表明，棕榈酸钠降低细胞活力，增加细胞内 TG 含量、脂滴积累、LPO 和 MDA 浓度，激活 caspase-3 和 caspase-9，导致细胞色素 c 从线粒体释放到细胞质，引发细胞凋亡。小檗碱可改善细胞内脂滴堆积和氧化应激，同时减少由棕榈酸钠诱导的细胞凋亡。
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