从中国辽宁黄檗中分离出的马铃薯Y病毒科一个新成员的全基因组序列。
Complete genome sequence of a novel member of the family Potyviridae isolated from Phellodendron amurense Rupr. in Liaoning, China.
作者信息
Han Tong, Hou Qiushi, Cheng Xiaofei, Garcia Juan Antonio, Valli Adrian, Zhang Song, Cao Mengji, Yang Caixia
机构信息
Liaoning Key Laboratory of Urban Integrated Pest Management and Ecological Security, College of Life Science and Engineering, Shenyang University, Dadong, Shenyang, 110044, Liaoning, China.
College of Agriculture, Northeast Agricultural University, Harbin, 150030, Heilongjiang, China.
出版信息
Arch Virol. 2019 Jun;164(6):1705-1709. doi: 10.1007/s00705-019-04210-3. Epub 2019 Mar 18.
In September 2017, Phellodendron amurense Rupr. plants showing yellow ringspots on leaves were observed in Liaoning, China. Flexuous filamentous particles (~1000 × 13 nm) were observed in the sap prepared from symptomatic leaves. A virus was detected in the symptomatic leaves by sequencing small RNAs and assembling the genome sequence. The complete genomic RNA was found to be 10,457 nucleotides in length excluding the poly(A) tail and to have the closest phylogenetic relationship to rose yellow mosaic virus (RoYMV), the sole member of newly established genus Roymovirus in the family Potyviridae. The coat protein gene (CP) of this virus shares 49.2% nucleotide and 55.1% amino acid sequence identity with that of RoYMV. These results suggest that this virus, which was named "phellodendron yellow ringspot-associated virus" (PYRaV) is a new member of the genus Roymovirus.
2017年9月，在中国辽宁观察到黄檗植株叶片上出现黄色环斑。从有症状叶片制备的汁液中观察到弯曲的丝状颗粒（约1000×13纳米）。通过对小RNA进行测序并组装基因组序列，在有症状的叶片中检测到一种病毒。发现完整的基因组RNA长度为10457个核苷酸，不包括聚腺苷酸尾，并且与玫瑰黄花叶病毒（RoYMV）具有最密切的系统发育关系，RoYMV是弹状病毒科新建立的Roymovirus属的唯一成员。该病毒的外壳蛋白基因（CP）与RoYMV的外壳蛋白基因在核苷酸水平上具有49.2%的序列同一性，在氨基酸水平上具有55.1%的序列同一性。这些结果表明，这种被命名为“黄檗黄环斑相关病毒”（PYRaV）的病毒是Roymovirus属的一个新成员。
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