Hou Qiushi, Han Tong, Li Liang, Wang Junzhu, Yu Meichun, Zhang Song, Cao Mengji, Yang Caixia
Liaoning Key Laboratory of Urban Integrated Pest Management and Ecological Security, College of Life Science and Engineering, Shenyang University, Dadong, Shenyang, 110044, Liaoning, China.
National Citrus Engineering and Technology Research Center, Citrus Research Institute, Southwest University, Beibei, Chongqing, 400712, China.
Arch Virol. 2019 May;164(5):1245-1248. doi: 10.1007/s00705-019-04220-1. Epub 2019 Mar 28.
In September 2017, a yellow spot leaf disease was noted on the leaves of Prunus davidiana (Carr.) Franch. plants in Liaoning, China, and spherical virions (approx. 30 nm in diameter) were later observed in preparations of symptomatic leaves. Subsequent deep sequencing of small RNA revealed the presence of a virus in these symptomatic leaves The complete genome of this viral isolate consists of 6,072 nucleotides, excluding the poly(A) tail. The virus showed the closest genetic relationship to grapevine-associated tymo-like virus, reported in Colmar, France (GaTLV, MH383239), which is the sole member of the newly proposed genus "Gratylivirus" within the order Tymovirales, which is currently unassigned to a particular family. The virus clustered closely with GaTLV in a phylogenetic tree constructed based on complete genomic sequences. On the basis of the nucleotide and amino acid sequences of the replicase and coat protein genes, this virus shares the highest (although still relatively low) sequence similarity with those of GaTLV (41.6%-60.8% identity), indicating that the virus is a distinct member of the order Tymovirales, for which the name "prunus yellow spot-associated virus" (PYSaV) is proposed. To our knowledge, this is the first report of a virus naturally infecting P. davidiana.
2017年9月，在中国辽宁的山桃（Prunus davidiana (Carr.) Franch.）植株叶片上发现了黄斑叶病，随后在有症状叶片的提取物中观察到球形病毒粒子（直径约30纳米）。随后对小RNA进行的深度测序显示，这些有症状的叶片中存在一种病毒。该病毒分离株的完整基因组由6072个核苷酸组成，不包括多聚（A）尾。该病毒与在法国科尔马报道的葡萄相关类番茄病毒（GaTLV，MH383239）显示出最密切的亲缘关系，GaTLV是新提出的“Gratylivirus”属的唯一成员，该属在番茄病毒目中，目前未被归入特定的科。在基于完整基因组序列构建的系统发育树中，该病毒与GaTLV紧密聚类。基于复制酶和外壳蛋白基因的核苷酸和氨基酸序列，该病毒与GaTLV的序列相似性最高（尽管仍然相对较低）（同一性为41.6%-60.8%），表明该病毒是番茄病毒目的一个独特成员，为此提出了“山桃黄斑相关病毒”（PYSaV）这一名称。据我们所知，这是关于一种自然感染山桃的病毒的首次报道。
