Bhowmik Deepshikha, Chakraborty Pinki, Bhowmick Rituparna, Sarkar Rakesh, Chetri Shiela, Bhattacharjee Amitabha
Department of Microbiology, Assam University, Silchar, Assam, India.
Indian J Med Microbiol. 2018 Oct-Dec;36(4):522-525. doi: 10.4103/ijmm.IJMM_18_285.
Coagulase Negative Staphylococci, the most commonly isolated pathogen are becoming emerging threats to the community as well as to the nosocomial environment. The present study underscores the distribution of Staphylococcal cassette chromosome mec (SCCmec) types among Methicillin resistant Coagulase Negative Staphylococci from the environmental origin.
Environmental and food sample (n = 460) from different location of northeastern region of India were collected for a period of one year and were phenotypically and genotypically screened using cefoxitin disc and PCR techniques for mecA and mecC gene detection. All the MR-CoNS isolates possessing mecA gene were subjected to 16srDNA sequencing for species identification. SCCmec typing was determined by evaluating using primer sets from type I to type V. Antibiotic susceptibility testing was performed for all the isolates. Statistical analysis with chi-square test using SPSS-21 statistical software.
Methicillin resistance shown by one hundred forty three isolates were carried out for molecular analysis, among them 53.84% serves as mecA carrier. Distribution of Staphylococcus haemolyticus was more frequent and was found that SCCmec types II and V were predominant among the study isolates. Linezolid was the drug of choice for the CoNS isolates. Statistical analysis showed an insignificant result for the tested antibiotics and SCCmec types.
This study therefore interprets the relative importance of SCCmec types among MR-CoNS isolates.
凝固酶阴性葡萄球菌是最常分离出的病原体，正成为社区以及医院环境中日益严重的威胁。本研究强调了环境来源的耐甲氧西林凝固酶阴性葡萄球菌中葡萄球菌盒式染色体mec（SCCmec）类型的分布情况。
在印度东北地区不同地点收集了为期一年的环境和食品样本（n = 460），并使用头孢西丁纸片法以及用于检测mecA和mecC基因的PCR技术进行表型和基因型筛选。所有携带mecA基因的耐甲氧西林凝固酶阴性葡萄球菌分离株都进行了16srDNA测序以进行菌种鉴定。通过使用I型至V型引物组进行评估来确定SCCmec分型。对所有分离株进行了抗生素敏感性测试。使用SPSS - 21统计软件进行卡方检验的统计分析。
对143株显示出耐甲氧西林的菌株进行了分子分析，其中53.84%为mecA携带者。溶血葡萄球菌的分布更为频繁，并且发现SCCmec II型和V型在研究分离株中占主导地位。利奈唑胺是凝固酶阴性葡萄球菌分离株的首选药物。统计分析显示，所测试的抗生素与SCCmec类型之间的结果无显著差异。
因此，本研究解释了SCCmec类型在耐甲氧西林凝固酶阴性葡萄球菌分离株中的相对重要性。
