Department of Immunology, Microbiology and Physiological Chemistry Faculty of Biotechnology and Animal Husbandry West Pomeranian University of Technology, Szczecin, Poland Doktora Judyma 24, 71-466, Szczecin, Poland.
J Microbiol. 2012 Jun;50(3):444-51. doi: 10.1007/s12275-012-1550-6. Epub 2012 Jun 30.
The aim of this study was an analysis of the staphylococcal flora of the nasal cavity of 42 healthy horses from 4 farms, along with species identification of CoNS isolates and determination of resistance to 18 antimicrobial agents, particularly phenotypic and genotypic methicillin resistance. From the 81 swabs, 87 staphylococci were isolated. All isolates possessed the gap gene but the coa gene was not detected in any of these isolates. Using PCR-RFLP of the gap gene, 82.8% of CoNS were identified: S. equorum (14.9%), S. warneri (14.9%), S. sciuri (12.6%), S. vitulinus (12.6%), S. xylosus (11.5%), S. felis (5.7%), S. haemolyticus (3.4%), S. simulans (3.4%), S. capitis (1.1%), S. chromogenes (1.1%), and S. cohnii subsp. urealyticus (1.1%). To our knowledge, this was the first isolation of S. felis from a horse. The species identity of the remaining Staphylococcus spp. isolates (17.2%) could not be determined from the gap gene PCR-RFLP analysis and 16S rRNA gene sequencing data. Based on 16S-23S intergenic transcribed spacer PCR, 11 different ITS-PCR profiles were identified for the 87 analyzed isolates. Results of API Staph were consistent with molecular identification of 17 (19.5%) isolates. Resistance was detected to only 1 or 2 of the 18 antimicrobial agents tested in the 17.2% CoNS isolates, including 6.9% MRCoNS. The mecA gene was detected in each of the 5 (5.7%) phenotypically cefoxitin-resistant isolates and in 12 (13.8%) isolates susceptible to cefoxitin. In total, from 12 horses (28.6%), 17 (19.5%) MRCoNS were isolated. The highest percentage of MRCoNS was noted among S. sciuri isolates (100%).
本研究旨在分析来自 4 个农场的 42 匹健康马鼻腔的葡萄球菌菌群，并对凝固酶阴性葡萄球菌（CoNS）分离株进行种属鉴定和对 18 种抗菌药物的耐药性检测，特别是表型和基因型耐甲氧西林。从 81 个拭子中分离出 87 株葡萄球菌。所有分离株均携带 g a p 基因，但未在这些分离株中检测到 coa 基因。通过 g a p 基因 PCR-RFLP，鉴定出 82.8%的 CoNS：马葡萄球菌（14.9%）、华纳葡萄球菌（14.9%）、松鼠葡萄球菌（12.6%）、牛葡萄球菌（12.6%）、木糖葡萄球菌（11.5%）、猫葡萄球菌（5.7%）、溶血葡萄球菌（3.4%）、模仿葡萄球菌（3.4%）、头状葡萄球菌（1.1%）、变绿气球菌（1.1%）和解脲支原体亚种（1.1%）。据我们所知，这是首次从马身上分离出猫葡萄球菌。剩余的葡萄球菌属（17.2%）分离株的种属身份无法通过 g a p 基因 PCR-RFLP 分析和 16S rRNA 基因测序数据确定。基于 16S-23S 基因间转录间隔区 PCR，对 87 株分析分离株鉴定出 11 种不同的 ITS-PCR 图谱。API Staph 的结果与 17 株（19.5%）分离株的分子鉴定结果一致。在 17.2% CoNS 分离株中，仅检测到 18 种抗菌药物中的 1 种或 2 种耐药，包括 6.9%的耐甲氧西林凝固酶阴性葡萄球菌（MRCoNS）。5 株（5.7%）表型头孢西丁耐药分离株和 12 株（13.8%）头孢西丁敏感分离株均检测到 mecA 基因。总共有 17 株（19.5%）MRCoNS 从 12 匹马（28.6%）中分离出来。在松鼠葡萄球菌分离株中，MRCoNS 的比例最高（100%）。
