Shandong Provincial Key Laboratory of Oral Tissue Regeneration, School of Stomatology, Shandong University, Jinan, People's Republic of China,
Department of Orthodontics, School of Stomatology, Shandong University, Jinan, People's Republic of China,
Int J Nanomedicine. 2019 Mar 11;14:1849-1863. doi: 10.2147/IJN.S190954. eCollection 2019.
Despite titanium (Ti) implants have been commonly used in the medical device field due to their superior biocompatibility, implant-associated bacterial infection remains a major clinical complication. Nanosilver, an effective antibacterial agent against a wide spectrum of bacterial strains, with a low-resistance potential, has attracted much interest too. Incorporation of nanosilver on Ti implants may be a promising approach to prevent biofilm formation.
The objective of the study was to investigate the antibacterial effects and osteoinductive properties of nanosilver/poly (dl-lactic-co-glycolic acid)-coated titanium (NSPTi).
Gram-positive methicillin-resistant (MRSA) and the Gram-negative opportunistic pathogen (PAO-1) were used to evaluate the antibacterial activity of NSPTi implants through the analysis of bacterial colonization in vitro and in vivo. Furthermore, we examined the osteoinductive potential of NSPTi implants by investigating the proliferation and differentiation of MC3T3-E1 preosteoblast cells. In vivo, the osteoinductive properties of NSPTi implants were assessed by radiographic evaluation, H&E staining, and Masson's trichrome staining.
In vitro, bacterial adhesion to the 2% NSPTi was significantly inhibited and <1% of adhered bacteria survived after 24 hours. In vitro, the average colony-forming units (CFU)/g ratios in the 2% NSPTi with 10 CFU MRSA and PAO-1 were 1.50±0.68 and 1.75±0.6, respectively. In the uncoated Ti groups, the ratios were 1.03±0.82×10 and 0.94±0.49×10, respectively. These results demonstrated that NSPTi implants had prominent antibacterial properties. Proliferation of MC3T3-E1 cells on the 2% NSPTi sample was 1.51, 1.78, and 2.22 times that on the uncoated Ti control after 3, 5, and 7 days' incubation, respectively. Furthermore, NSPTi implants promoted the maturation and differentiation of MC3T3-E1 cells. In vivo, NSPTi accelerated the formation of new bone while suppressing bacterial survival.
NSPTi implants have simultaneous antibacterial and osteoinductive activities and therefore have the potential in clinical applications.
尽管钛（Ti）植入物由于其优异的生物相容性而在医疗器械领域得到了广泛应用，但植入物相关的细菌感染仍然是一个主要的临床并发症。纳米银作为一种对多种细菌菌株都具有有效抗菌作用的抗生素，其耐药潜力较低，也引起了广泛关注。将纳米银掺入 Ti 植入物中可能是预防生物膜形成的一种有前途的方法。
本研究旨在探讨纳米银/聚（DL-乳酸-共-乙醇酸）涂层钛（NSPTi）的抗菌效果和骨诱导性能。
采用革兰氏阳性耐甲氧西林金黄色葡萄球菌（MRSA）和革兰氏阴性机会致病菌（PAO-1），通过体外和体内细菌定植分析，评估 NSPTi 植入物的抗菌活性。此外，通过研究 MC3T3-E1 前成骨细胞的增殖和分化，研究 NSPTi 植入物的骨诱导潜力。体内实验通过影像学评估、H&E 染色和 Masson 三色染色评估 NSPTi 植入物的骨诱导特性。
体外实验中，2% NSPTi 显著抑制细菌黏附，24 小时后黏附细菌存活率<1%。体外实验中，10 CFU MRSA 和 PAO-1 接种于 2% NSPTi 后，其平均菌落形成单位（CFU）/g 比值分别为 1.50±0.68 和 1.75±0.6，而未涂层 Ti 组的比值分别为 1.03±0.82×10 和 0.94±0.49×10。这些结果表明 NSPTi 植入物具有显著的抗菌性能。MC3T3-E1 细胞在 2% NSPTi 样本上的增殖分别是未涂层 Ti 对照组的 1.51、1.78 和 2.22 倍，培养 3、5 和 7 天后。此外，NSPTi 植入物促进了 MC3T3-E1 细胞的成熟和分化。体内实验中，NSPTi 加速了新骨的形成，同时抑制了细菌的存活。
NSPTi 植入物具有抗菌和骨诱导的双重活性，因此具有在临床应用中的潜力。
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