AgBr-NP@CTMAB 杂化的合成、抗真菌活性和细胞毒性及其在 PMMA 中的应用。
Synthesis, Antifungal Activity, and Cytotoxicity of AgBr-NP@CTMAB Hybrid and Its Application in PMMA.
机构信息
Fujian Key Laboratory of Oral Diseases, Fujian Biological Materials Engineering and Technology Center of Stomatology, School and Hospital of Stomatology, Fujian Medical University, Fuzhou, Fujian, 350004, People's Republic of China.
Department of Prosthodontics, School and Hospital of Stomatology, Fujian Medical University, Fuzhou, 350001, People's Republic of China.
出版信息
Int J Nanomedicine. 2021 Apr 29;16:3091-3103. doi: 10.2147/IJN.S290673. eCollection 2021.
OBJECTIVE
To synthesize and determine the antifungal activity of AgBr-nanoparticles (NP) @CTMAB (cetyltrimethyl-ammonium bromide) against () for use in the field of denture cleaning.
METHODS
The morphology and structure of AgBr-NP@CTMAB were characterized by IR, UV-Vis, XRD and SEM. The antifungal potential of AgBr-NP@CTMAB against was determined by colony formation assay and growth curve analysis. PMMA containing AgBr-NP@CTMAB was prepared, and the long-term antifungal efficacy was analyzed. The effect against biofilm was analyzed by SEM and OD600 , and the color changes of the specimens were observed by stereomicroscopy after 1 week of incubation. Cytotoxicity to human oral gingival fibroblasts and oral mucosal epithelial cells was detected by Cell Counting Kit-8 (CCK-8) in vitro.
RESULTS
The compound showed a good crystalline phase, the presence of AgBr nanoparticles and the hybridization of CTMAB+ with AgBr-NPs. AgBr-NP@CTMAB showed significant antifungal activity against at concentrations of 10 μg/mL and 20 μg/mL. PMMA specimens containing AgBr-NP@CTMAB showed no long-term antifungal effect against biofilm. The clearance rate of attached to PMMA was 44.73% after soaking in 10 µg/mL AgBr-NP@CTMAB solution for 30 min and 91.35% for 8 h. There was no significant residual cytotoxicity or visual color change after soaking.
SIGNIFICANCE
AgBr-NP@CTMAB showed promising potential treatment for denture cleaners.
目的
合成并测定 AgBr-纳米粒子（NP）@CTMAB（十六烷基三甲基溴化铵）对 的抗真菌活性，以期将其应用于义齿清洁领域。
方法
采用红外光谱（IR）、紫外-可见光谱（UV-Vis）、X 射线衍射（XRD）和扫描电子显微镜（SEM）对 AgBr-NP@CTMAB 的形态和结构进行了表征。采用集落形成实验和生长曲线分析测定了 AgBr-NP@CTMAB 对 的抗真菌潜力。制备含 AgBr-NP@CTMAB 的聚甲基丙烯酸甲酯（PMMA），并分析其长期抗真菌效果。通过 SEM 和 OD600 分析了对 生物膜的影响，并通过体视显微镜观察了孵育 1 周后标本的颜色变化。通过细胞计数试剂盒-8（CCK-8）法体外检测对人口腔牙龈成纤维细胞和口腔黏膜上皮细胞的细胞毒性。
结果
该化合物具有良好的结晶相，存在 AgBr 纳米颗粒和 CTMAB+与 AgBr-NPs 的杂交。AgBr-NP@CTMAB 在浓度为 10 μg/mL 和 20 μg/mL 时对 表现出显著的抗真菌活性。含 AgBr-NP@CTMAB 的 PMMA 标本对 生物膜无长期抗真菌作用。浸泡在 10 µg/mL AgBr-NP@CTMAB 溶液 30 分钟后，附着在 PMMA 上的 清除率为 44.73%，8 小时后为 91.35%。浸泡后无明显残留细胞毒性或肉眼可见颜色变化。
意义
AgBr-NP@CTMAB 显示出对义齿清洁剂有良好的治疗潜力。
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