Uppuluri Priya, Busscher Henk J, Chakladar Jaideep, van der Mei Henny C, Chaffin W LaJean
Division of Infectious Diseases, Los Angeles Biomedical Research Institute at Harbor-University of California Los Angeles Medical Center, TorranceTorrance, CA, United States.
Department of Biomedical Engineering, University of Groningen and University Medical Center GroningenGroningen, Netherlands.
Front Cell Infect Microbiol. 2017 Jul 13;7:311. doi: 10.3389/fcimb.2017.00311. eCollection 2017.
Biofilms on silicone rubber voice prostheses are the major cause for frequent failure and replacement of these devices. The presence of both bacterial and yeast strains has been suggested to be crucial for the development of voice prosthetic biofilms. Polymicrobial biofilms that include and are the leading cause of voice prosthesis failure. An biofilm comprising these two organisms was developed on silicone rubber, a material used for Groningen button voice prosthesis. We found that this biofilm environment was not conducive for growth or differentiation. Global transcriptional analyses of biofilm cells grown with revealed that genes with functions related to cell cycle progression and hyphal development were repressed >2-fold. The mixed species biofilms were more compact and less robust compared to mono-species biofilms, even when developed under conditions of continuous nutrient flow. Under these conditions also significantly inhibited biofilm dispersal. Preferential adherence of to candidal hyphae was mediated by the adhesin Als3.
硅橡胶语音假体上的生物膜是这些装置频繁失效和更换的主要原因。细菌和酵母菌株的存在被认为对语音假体生物膜的形成至关重要。包含[具体细菌名称1]和[具体细菌名称2]的多微生物生物膜是语音假体失效的主要原因。在用于格罗宁根纽扣语音假体的硅橡胶材料上形成了包含这两种微生物的生物膜。我们发现这种生物膜环境不利于[具体细菌名称1]的生长或分化。对与[具体细菌名称2]一起生长的[具体细菌名称1]生物膜细胞进行的全局转录分析表明，与细胞周期进程和菌丝发育相关功能的基因被抑制超过2倍。与[具体细菌名称1]单物种生物膜相比，混合物种生物膜更致密但更脆弱，即使在连续营养流条件下形成也是如此。在这些条件下，[具体细菌名称2]也显著抑制[具体细菌名称1]生物膜的分散。[具体细菌名称2]对念珠菌菌丝的优先粘附由粘附素Als3介导。
