VHS107基因家族在X连锁免疫缺陷缺陷小鼠的脂多糖敏感B细胞亚群中表达缺失。
Lack of expression of the VHS107 gene family in the lipopolysaccharide-sensitive B cell subset of X-linked immunodeficiency-defective mice.
作者信息
Primi D, Cazenave P A
出版信息
J Exp Med. 1986 Jul 1;164(1):357-62. doi: 10.1084/jem.164.1.357.
The expression of the VH T15 gene product was analyzed in the sera and in the supernatants of polyclonally activated B cells of X-linked immunodeficient (xid) mice. We found that defective males, contrary to normal females, do not express the VH T15 gene product even on antibodies devoid of anti-PC specificity. RNA analysis of polyclonally activated cells with VH specific probes revealed that xid-defective B cells do not express the entire S107 or part of the J606 VH family. Members of the J558 family, on the other hand, are equally well expressed among defective males and normal female B cells. These results strongly suggest that VH families are asymmetrically represented among B cell subsets.
在X连锁免疫缺陷（xid）小鼠的血清以及多克隆激活的B细胞上清液中分析了VH T15基因产物的表达。我们发现，与正常雌性小鼠相反，有缺陷的雄性小鼠即使在缺乏抗PC特异性的抗体上也不表达VH T15基因产物。用VH特异性探针分析多克隆激活细胞的RNA显示，xid缺陷的B细胞不表达整个S107或J606 VH家族的一部分。另一方面，J558家族的成员在有缺陷的雄性B细胞和正常雌性B细胞中表达情况相同。这些结果有力地表明，VH家族在B细胞亚群中的表达是不对称的。
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