Beijing National Laboratory for Condensed-Matter Physics and Institute of Physics, Chinese Academy of Sciences, Beijing 100190, China.
Nanoscale. 2019 Mar 28;11(13):6263-6269. doi: 10.1039/c8nr10474c.
Tetrahedral DNA nanostructures (TDNs) are programmable DNA nanostructures that have great potential in bio-sensing, cell imaging and therapeutic applications. In this study, we investigate the translocation behavior of individual TDNs through solid-state nanopores. Pronounced translocation signals for TDNs are observed that are sensitive to the size of the nanostructures. TDNs bound to linear DNA molecules produce an extra signal in the ionic current traces. Statistical analysis of its relative temporal position reveals distinct features between TDNs bound to the end and those bound to the middle of the linear DNA molecules. A featured current trace for two TDNs bound to the same linear DNA molecule has also been observed. Our study demonstrates the potential of using TDNs as sensitive bio-sensors to detect specific segments of a single DNA molecule in real time, based on solid-state nanopore devices.
四面体形 DNA 纳米结构（TDN）是可编程的 DNA 纳米结构，在生物传感、细胞成像和治疗应用方面具有巨大的潜力。在这项研究中，我们研究了单个 TDN 通过固态纳米孔的迁移行为。观察到 TDN 的明显迁移信号对纳米结构的大小敏感。与线性 DNA 分子结合的 TDN 在离子电流迹中产生额外的信号。对其相对时间位置的统计分析揭示了与线性 DNA 分子末端结合的 TDN 和与线性 DNA 分子中间结合的 TDN 之间的明显特征。还观察到了与同一线性 DNA 分子结合的两个 TDN 的特征电流迹。我们的研究表明，基于固态纳米孔器件，TDN 作为灵敏的生物传感器，有可能实时检测单个 DNA 分子的特定片段。
