Chongqing School, University of Chinese Academy of Sciences, Beijing, China; Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Sciences, Beijing, China.
Chongqing School, University of Chinese Academy of Sciences, Beijing, China; Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Sciences, Beijing, China; Changchun University of Science and Technology, Changchun, China.
Bioelectrochemistry. 2023 Feb;149:108284. doi: 10.1016/j.bioelechem.2022.108284. Epub 2022 Oct 8.
In this paper, a novel detection technique for tumor marker carcinoembryonic antigen (CEA) has been developed by using a solid-state nanopore as a tool. The system utilizes the specific affinity between aptamer-modified magnetic FeO and CEA, rather than directly detecting the translocation of CEA through the nanopore. The aptamer-modified magnetic FeO was hybridized with tetrahedral DNA nanostructures (TDNs), and TDNs were released after CEA was added. We investigate the translocation behavior of individual TDNs through solid-state nanopores. The frequency of the blockage signals for TDNs is recorded for indirect detection of CEA. We realized the detection of CEA with a concentration as low as 0.1 nM and proved the specificity of the interaction between the aptamer. In addition, our designed nanopore sensing strategy can detect CEA in real samples.
本文提出了一种利用固态纳米孔作为工具检测肿瘤标志物癌胚抗原（CEA）的新方法。该系统利用适配体修饰的磁性 FeO 与 CEA 之间的特异性亲和力，而不是直接通过纳米孔检测 CEA 的易位。将适配体修饰的磁性 FeO 与四面体 DNA 纳米结构（TDN）杂交，加入 CEA 后释放 TDN。我们研究了单个 TDN 通过固态纳米孔的易位行为。记录 TDN 的阻塞信号频率，以间接检测 CEA。我们实现了对浓度低至 0.1 nM 的 CEA 的检测，并证明了适配体之间相互作用的特异性。此外，我们设计的纳米孔传感策略可以检测真实样本中的 CEA。
