Department of Molecular Biology, Princeton University, Princeton, NJ, USA.
Department of Plant Biology, Carnegie Institution for Science, Stanford, CA, USA.
Nat Genet. 2019 Apr;51(4):627-635. doi: 10.1038/s41588-019-0370-6. Epub 2019 Mar 18.
Photosynthetic organisms provide food and energy for nearly all life on Earth, yet half of their protein-coding genes remain uncharacterized. Characterization of these genes could be greatly accelerated by new genetic resources for unicellular organisms. Here we generated a genome-wide, indexed library of mapped insertion mutants for the unicellular alga Chlamydomonas reinhardtii. The 62,389 mutants in the library, covering 83% of nuclear protein-coding genes, are available to the community. Each mutant contains unique DNA barcodes, allowing the collection to be screened as a pool. We performed a genome-wide survey of genes required for photosynthesis, which identified 303 candidate genes. Characterization of one of these genes, the conserved predicted phosphatase-encoding gene CPL3, showed that it is important for accumulation of multiple photosynthetic protein complexes. Notably, 21 of the 43 higher-confidence genes are novel, opening new opportunities for advances in understanding of this biogeochemically fundamental process. This library will accelerate the characterization of thousands of genes in algae, plants, and animals.
光合生物为地球上几乎所有的生命提供食物和能量，但它们的一半蛋白质编码基因仍未被描述。通过单细胞生物的新遗传资源，这些基因的描述可以大大加快。在这里，我们为单细胞藻类莱茵衣藻生成了一个全基因组、映射插入突变体的索引文库。该文库中的 62389 个突变体覆盖了 83%的核蛋白编码基因，可供社区使用。每个突变体都包含独特的 DNA 条码，允许将该文库作为一个整体进行筛选。我们对光合作用所需的基因进行了全基因组调查，鉴定出 303 个候选基因。对其中一个基因，保守的预测磷酸酶编码基因 CPL3 的特征描述表明，它对多种光合蛋白复合物的积累很重要。值得注意的是，43 个高可信度基因中有 21 个是新的，为理解这一具有生物地球化学基础的过程提供了新的机会。这个文库将加速藻类、植物和动物中数千个基因的描述。
Zotero 插件
