Michaelis G, Vahrenholz C, Pratje E
Botanisches Institut der Universität Düsseldorf, Federal Republic of Germany.
Mol Gen Genet. 1990 Sep;223(2):211-6. doi: 10.1007/BF00265056.
We have sequenced the termini of the mitochondrial genome of Chlamydomonas reinhardtii and now present the DNA sequence of the gene for apocytochrome b. This gene is the thirteenth gene of the linear 15.8 kb DNA and appears to be the last one of the mt genome. The deduced protein sequence of 381 amino acid residues shows 56%, 48.6% and 48% identity with the apocytochrome b proteins of maize, Drosophila yakuba and mouse, respectively. RNA analysis reveals a transcript of about 1250 nucleotides. It is now possible to present the complete protein-coding capacity, the pattern of codon utilization for all eight protein genes, and the complete functional map of the mitochondrial 15.8 kb DNA of C. reinhardtii. One surprising feature is the absence of mitochondrial genes for ATPase and subunits II and III of cytochrome oxidase. No more than three tRNA genes appear to be present on the 15.8 kb mitochondrial DNA.
我们已对莱茵衣藻线粒体基因组的末端进行了测序，现在展示脱辅基细胞色素b基因的DNA序列。该基因是线性15.8 kb DNA的第13个基因，似乎是线粒体基因组的最后一个基因。推导的由381个氨基酸残基组成的蛋白质序列与玉米、雅库布果蝇和小鼠的脱辅基细胞色素b蛋白分别具有56%、48.6%和48%的同一性。RNA分析揭示了一个约1250个核苷酸的转录本。现在可以展示莱茵衣藻线粒体15.8 kb DNA完整的蛋白质编码能力、所有八个蛋白质基因的密码子使用模式以及完整的功能图谱。一个令人惊讶的特征是缺乏ATP酶以及细胞色素氧化酶亚基II和III的线粒体基因。在15.8 kb的线粒体DNA上似乎存在不超过三个tRNA基因。
