Department of Environmental Engineering, Faculty of Engineering, Munzur University, TR62000, Tunceli, Turkey.
Department of Food Process, Vocational School, Munzur University, Tunceli, Turkey.
Environ Sci Pollut Res Int. 2019 May;26(13):12807-12812. doi: 10.1007/s11356-019-04813-y. Epub 2019 Mar 18.
In order to evaluate the potential ecological risk and the toxic effect of landfill leachate (LL), Phanerochaete chrysosporium was exposed to LL and their biochemical response was observed by using antioxidant parameters. Phanerochaete chrysosporium, ME 446, was kept at 4 °C after being sub-cultured at 28 °C on Sabouraud Dextrose Agar (SDA). Superoxide dismutase (SOD), catalase (CAT) activities, and malaondialdehyde (MDA) and glutathione (GSH) levels of P. chrysosporium exposed to different dilution rates of leachate (1/10 and 1/20) for 24 and 96 h were analyzed by using the ELISA method. The physiochemical parameters such as pH, conductivity, total dissolved solids (TDS), dissolved oxygen (DO), chemical oxygen demand (COD) of leachate, and reference water were analyzed by using the YSI Professional Plus handheld multiparameter meter. In this study, SOD activities were decreased in the application groups compared with the Control Group at the 24th and 96th hours. CAT activities and GSH levels increased in the application groups compared with the Control Group at the 24th hour but decreased at the 96th hours. MDA levels increased in all of the application groups when compared with the Control Group for both 24 and 96 h. Different concentration of LL induces oxidative stress in P. chrysosporium, increased CAT activity and MDA levels, and decreased SOD activity and GSH levels.
为了评估垃圾渗滤液（LL）的潜在生态风险和毒性效应，采用抗氧化参数观察黄孢原毛平革菌（Phanerochaete chrysosporium）暴露于 LL 时的生化反应。Phanerochaete chrysosporium，ME 446，在 28°C 的萨布罗琼脂（SDA）上继代培养后，保存在 4°C。采用 ELISA 法分析了不同稀释率（1/10 和 1/20）的渗滤液（1/10 和 1/20）暴露 24 和 96 小时后，黄孢原毛平革菌的超氧化物歧化酶（SOD）、过氧化氢酶（CAT）活性、丙二醛（MDA）和谷胱甘肽（GSH）水平。采用 YSI Professional Plus 手持式多参数水质仪分析渗滤液和参比水的理化参数，如 pH 值、电导率、总溶解固体（TDS）、溶解氧（DO）、化学需氧量（COD）。在这项研究中，与对照组相比，应用组在 24 小时和 96 小时时 SOD 活性降低。与对照组相比，应用组在 24 小时时 CAT 活性和 GSH 水平增加，但在 96 小时时 CAT 活性和 GSH 水平降低。与对照组相比，在 24 小时和 96 小时时，所有应用组的 MDA 水平均升高。不同浓度的 LL 会导致黄孢原毛平革菌产生氧化应激，增加 CAT 活性和 MDA 水平，降低 SOD 活性和 GSH 水平。
