College of Public Health, University of South China, Hengyang, 421001, People's Republic of China.
Mikrochim Acta. 2019 Mar 19;186(4):250. doi: 10.1007/s00604-019-3360-6.
It is shown that metallothionein-stabilized copper nanoclusters (MT-CuNCs) display catalase-like activity. In the presence of either lead(II) or mercury(II), the catalase-like activity is converted to a peroxidase-like activity. On addition of Pb(II) or Hg(II), the inhibitory effect of MT-CuNCs on the chromogenic reaction of 3,3',5,5'-tetramethylbenzidine (TMB) with HO is weakened. On the other hand, the catalytic effect of the nanoclusters on the chromogenic reaction is increased. The system MT-CuNCs-Pb(II)/Hg(II) exhibits high affinity for the substrates TMB and HO. Their catalytic behavior follows Michaelis-Menten kinetics. Based on these findings, a method was developed for visual detection (via the blue coloration formed) and spectrophotometric determination (at 450 nm) of Pb(II) and Hg(II). The linear range for Pb(II) extends from 0.7 to 96 μM, and the linear ranges for Hg(II) from 97 nM to 2.3 μM and from 3.1 μM to 15.6 μM. The detection limits are 142 nM for Pb(II) and 43.8 nM for Hg(II). Graphical abstract Metallothionein-stabilized copper nanoclusters (MT-CuNCs) display catalase-like activity. On addition of Pb(II) or Hg(II), the catalase-like activity is converted to a peroxidase-like activity. The latter catalyzes the oxidation of 3,3',5,5'-tetramethylbenzidine (TMB) by HO.
金属硫蛋白稳定的铜纳米簇（MT-CuNCs）显示出过氧化氢酶样活性。在存在铅（II）或汞（II）的情况下，过氧化氢酶样活性转化为过氧化物酶样活性。加入 Pb(II) 或 Hg(II) 后，MT-CuNCs 对 3,3',5,5'-四甲基联苯胺（TMB）与 HO 的显色反应的抑制作用减弱。另一方面，纳米簇对显色反应的催化作用增强。MT-CuNCs-Pb(II)/Hg(II) 体系对 TMB 和 HO 底物具有高亲和力。它们的催化行为遵循米氏动力学。基于这些发现，开发了一种用于可视化检测（通过形成的蓝色）和分光光度法测定（在 450nm 处）Pb(II) 和 Hg(II) 的方法。Pb(II) 的线性范围从 0.7 到 96μM，Hg(II) 的线性范围从 97nM 到 2.3μM 和从 3.1μM 到 15.6μM。Pb(II) 的检测限为 142nM，Hg(II) 的检测限为 43.8nM。
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