State Key Laboratory of Food Nutrition and Safety, College of Food Engineering and Biotechnology , Tianjin University of Science and Technology , Tianjin 300457 , PR China.
Key Laboratory of Environmentally Friendly Chemistry and Applications of Ministry of Education, Department of Chemistry , Xiangtan University , Xiangtan , Hunan 411105 , PR China.
Anal Chem. 2018 Aug 7;90(15):9060-9067. doi: 10.1021/acs.analchem.8b01384. Epub 2018 Jul 18.
In this work, highly photoluminescent (PL) self-assembled copper nanoclusters (Cu NCs) capable of rapid, sensitive, and selective detection of histamine were developed. Cu NCs were synthesized in facile conditions by using 2,3,5,6-tetrafluorothiophenol (TFTP) as both the reducing agent and the protecting ligand, which exhibited intense saffron yellow (590 nm) PL via self-assembled induced emission (SAIE), and the absolute quantum yield (QY) of assembly was as high as 43.0%. The size, electronic states, and morphologies of the assembled nanoribbons were characterized, and the geometric structure and spectral properties of the Cu NCs were investigated by theoretical study. Furthermore, the mechanism of the excellent sensing performance of Cu NCs toward histamine was demonstrated by scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS), and energy dispersive X-ray analysis (EDX). With this sensing system, the amount of histamine in fish, shrimp, and red wine were analyzed, and experiment results verified the application of the sensor. Importantly, the luminescent test strips based on Cu NCs were fabricated for colorimetric detection of histamine in foods. This proposed technique may provide an alternative to traditional methods for histamine detection.
在这项工作中，开发了具有高荧光（PL）的自组装铜纳米团簇（Cu NCs），能够快速、灵敏和选择性地检测组氨酸。Cu NCs 在简便的条件下通过使用 2,3,5,6-四氟噻吩酚（TFTP）作为还原剂和保护配体来合成，通过自组装诱导发射（SAIE）表现出强烈的藏红花黄色（590nm）PL，组装的绝对量子产率（QY）高达 43.0%。对组装纳米带的尺寸、电子态和形态进行了表征，并通过理论研究研究了 Cu NCs 的几何结构和光谱性质。此外，通过扫描电子显微镜（SEM）、透射电子显微镜（TEM）、X 射线光电子能谱（XPS）和能谱分析（EDX）证明了 Cu NCs 对组氨酸具有优异的传感性能的机制。使用该传感系统分析了鱼、虾和红酒中的组氨酸含量，实验结果验证了传感器的应用。重要的是，基于 Cu NCs 的荧光测试条被用于食品中组氨酸的比色检测。该技术可能为组氨酸检测提供一种替代传统方法的方法。
