Drexler H G, Menon M, Minowada J
Acta Haematol. 1986;75(1):12-7. doi: 10.1159/000206072.
The expression of the enzyme marker terminal deoxynucleotidyl transferase (TdT) was examined by immunofluorescence assay in the cells from 333 cases with various types and subtypes of leukemia or lymphoma. More than 90% of cALL and T-ALL, 70% of Null-ALL and 80% of pre-B-ALL were TdT-positive. One case in the commonly TdT-negative group of B-ALL showed TdT-positive cells. All cases of mature B-cell malignancies (B-CLL, hairy cell leukemia, B-cell lymphoma) have been TdT-negative. In the group of mature T-cell malignancies, T-CLL and mycosis fungoides were negative and 2 out of 6 mature T-cell lymphomas were TdT-positive. 13% of acute myeloid leukemias and 36% of CML in blast crisis expressed TdT. Therefore, these TdT-positive cases of CML in blast crisis also carrying the common ALL-antigen belong to the lymphoid subtype. CML and erythroleukemia were invariably TdT-negative. TdT has become an indispensable indicator of immature lymphoid leukemia cells and is particularly valuable as part of the panel of markers used in leukemia phenotyping.
采用免疫荧光分析法检测了333例各种类型和亚型白血病或淋巴瘤患者细胞中酶标志物末端脱氧核苷酸转移酶（TdT）的表达情况。超过90%的普通型急性淋巴细胞白血病（cALL）和T细胞急性淋巴细胞白血病（T-ALL）、70%的无标记型急性淋巴细胞白血病（Null-ALL）和80%的前B细胞急性淋巴细胞白血病（pre-B-ALL）为TdT阳性。B-ALL通常为TdT阴性组中有1例显示TdT阳性细胞。所有成熟B细胞恶性肿瘤（B细胞慢性淋巴细胞白血病、毛细胞白血病、B细胞淋巴瘤）均为TdT阴性。在成熟T细胞恶性肿瘤组中，T细胞慢性淋巴细胞白血病（T-CLL）和蕈样肉芽肿为阴性，6例成熟T细胞淋巴瘤中有2例TdT阳性。13%的急性髓系白血病和36%的急变期慢性髓系白血病（CML）表达TdT。因此，这些急变期CML的TdT阳性病例也携带常见的急性淋巴细胞白血病抗原，属于淋巴样亚型。慢性髓系白血病和红白血病始终为TdT阴性。TdT已成为未成熟淋巴细胞白血病细胞不可或缺的指标，作为白血病表型分析所用标志物组合的一部分，其具有特别重要的价值。
