Ghosh P, Andrews J L, Osborne R A, Lesjak M S
Agents Actions Suppl. 1986;18:69-81. doi: 10.1007/978-3-0348-7684-1_10.
The proteoglycans, and proteinase inhibitors of normal and degenerate human articular cartilage have been isolated by 4.0M GuHCl extraction, CsCl density gradient ultracentrifugation and Sephadex G-75 gel filtration chromatography. While a small amount of trypsin inhibitory activity eluted in the Sephadex G-75 void volume, the major proteinase inhibitors were included in the column with MWs within the range 1 - 3 X 10(4) daltons. The large molecular weight trypsin inhibitor was identified as alpha 1-trypsin inhibitor, which was present in greater amounts in degenerate tissue. The Sephadex G75 included fractions (LMWCI) showed inhibition against cysteine and serine proteinases and were reduced in degenerate tissue, but slightly elevated in aged "normal" cartilage. We suggest that the resistance of ageing cartilage to enzymatic degradation may be related to the levels of LMWCI within these tissues.
通过4.0M盐酸胍提取、氯化铯密度梯度超速离心和葡聚糖凝胶G-75凝胶过滤色谱法，已分离出正常和退变人关节软骨中的蛋白聚糖和蛋白酶抑制剂。虽然少量胰蛋白酶抑制活性在葡聚糖凝胶G-75的空体积中洗脱，但主要的蛋白酶抑制剂包含在分子量范围为1 - 3×10⁴道尔顿的柱中。大分子胰蛋白酶抑制剂被鉴定为α1-胰蛋白酶抑制剂，其在退变组织中的含量更高。葡聚糖凝胶G75包含的组分（低分子量软骨抑制剂，LMWCI）对半胱氨酸和丝氨酸蛋白酶有抑制作用，在退变组织中减少，但在老化的“正常”软骨中略有升高。我们认为，老化软骨对酶促降解的抗性可能与这些组织中LMWCI的水平有关。
